Reynolds, Louise A, Hornett, Emily A 
ORCID: 0000-0002-7747-0583, Jiggins, Chris D and Hurst, Gregory DD ORCID: 0000-0002-7163-7784
(2019)
Suppression of Wolbachia-mediated male-killing in the butterfly Hypolimnas bolina involves a single genomic region
PEERJ, 7 (10).
e7677-.
ISSN 2167-8359, 2167-8359
Abstract
Background. Sex ratio distorting agents (maternally inherited symbionts and meiotically-driving sex chromosomes) are common in insects. When these agents rise to high frequencies they create strong population sex ratio bias and selection then favours mutations that act to restore the rare sex. Despite this strong selection pressure, the evolution of mutations that suppress sex ratio distorting elements appears to be constrained in many cases, where sex-biased populations persist for many generations. This scenario has been observed in the butterfly Hypolimnas bolina, where Wolbachia-mediated male killing endured for 800–1,000 generations across multiple populations before the evolution of suppression. Here we test the hypothesis that this evolutionary lag is the result of suppression being a multilocus trait requiring multiple mutations. Methods. We developed genetic markers, based on conservation of synteny, for each H. bolina chromosome and verified coverage using recombinational mapping. We then used a Wolbachia-infected mapping family to assess each chromosome for the presence of loci required for male survival, as determined by the presence of markers in all surviving sons. Results. Informative markers were obtained for each of the 31 chromosomes in H. bolina. The only marker that cosegregated with suppression was located on chromosome 25. A genomic region necessary for suppression has previously been located on this chromosome. We therefore conclude that a single genomic region of the H. bolina genome is necessary for male-killing suppression. Discussion. The evolutionary lag observed in our system is not caused by a need for changes at multiple genomic locations. The findings favour hypotheses in which either multiple mutations are required within a single genomic region, or the suppressor mutation is a singularly rare event.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Symbiosis, Coevolution, Selfish genetic elements, Wolbachia |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 10 Oct 2019 09:45 |
| Last Modified: | 16 Jun 2026 07:08 |
| DOI: | 10.7717/peerj.7677 |
| Open Access URL: | https://peerj.com/articles/7677/ |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3057691 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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