The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle



Nixon, Faye M, Gutierrez-Caballero, Cristina, Hood, Fiona E, Booth, Daniel G, Prior, Ian A ORCID: 0000-0002-4055-5161 and Royle, Stephen J
(2015) The mesh is a network of microtubule connectors that stabilizes individual kinetochore fibers of the mitotic spindle. ELIFE, 4.

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Abstract

Kinetochore fibers (K-fibers) of the mitotic spindle are force-generating units that power chromosome movement during mitosis. K-fibers are composed of many microtubules that are held together throughout their length. Here, we show, using 3D electron microscopy, that K-fiber microtubules (MTs) are connected by a network of MT connectors. We term this network ‘the mesh’. The K-fiber mesh is made of linked multipolar connectors. Each connector has up to four struts, so that a single connector can link up to four MTs. Molecular manipulation of the mesh by overexpression of TACC3 causes disorganization of the K-fiber MTs. Optimal stabilization of K-fibers by the mesh is required for normal progression through mitosis. We propose that the mesh stabilizes K-fibers by pulling MTs together and thereby maintaining the integrity of the fiber. Our work thus identifies the K-fiber meshwork of linked multipolar connectors as a key integrator and determinant of K-fiber structure and function.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 30 Oct 2019 08:53
Last Modified: 17 Oct 2021 02:13
DOI: 10.7554/eLife.07635
Open Access URL: https://elifesciences.org/articles/07635
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3059965