High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo

Aljabr, Waleed ORCID: 0000-0002-9385-2776, Armstrong, Stuart ORCID: 0000-0002-3862-1801, Rickett, Natasha Y, Pollakis, Georgios ORCID: 0000-0002-9659-5461, Touzelet, Olivier, Cloutman-Green, Elaine, Matthews, David A and Hiscox, Julian A ORCID: 0000-0002-6582-0275 (2019) High Resolution Analysis of Respiratory Syncytial Virus Infection In Vivo. VIRUSES-BASEL, 11 (10). E926-.

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Official URL: http://dx.doi.org/10.3390/v11100926

Abstract

Human respiratory syncytial virus (HRSV) is a major cause of pediatric infection and also causes disease in the elderly and those with underlying respiratory problems. There is no vaccine for HRSV and anti-viral therapeutics are not broadly applicable. To investigate the effect of HRSV biology in children, nasopharyngeal aspirates were taken from children with different viral loads and a combined high throughput RNAseq and label free quantitative proteomics approach was used to characterize the nucleic acid and proteins in these samples. HRSV proteins were identified in the nasopharyngeal aspirates from infected children, and their abundance correlated with viral load (Ct value), confirming HRSV infection. Analysis of the HRSV genome indicated that the children were infected with sub-group A virus and that minor variants in nucleotide frequency occurred in discrete clusters along the HRSV genome, and within a patient clustered distinctly within the glycoprotein gene. Data from the samples were binned into four groups; no-HRSV infection (control), high viral load (Ct < 20), medium viral load (Ct = 20-25), and low viral load (Ct > 25). Cellular proteins associated with the anti-viral response (e.g., ISG15) were identified in the nasopharyngeal aspirates and their abundance was correlated with viral load. These combined approaches have not been used before to study HRSV biology in vivo and can be readily applied to the study the variation of virus host interactions.

Item Type:	Article
Uncontrolled Keywords:	respiratory syncytial virus, proteomics, RNAseq, nasopharyngeal aspirate, host response, quasispecies, clinical sample, respiratory disease
Depositing User:	Symplectic Admin
Date Deposited:	04 Nov 2019 09:34
Last Modified:	19 Jan 2023 00:21
DOI:	10.3390/v11100926
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3060239