The development and validation of a novel LC-MS/MS method for the quantification of cenicriviroc in human plasma and cerebrospinal fluid



Penchala, Sujan Dilly, Alagaratnam, Jasmini, Challenger, Elizabeth ORCID: 0000-0002-8978-6067, Amara, Alieu ORCID: 0000-0002-1137-2948, Else, Laura, Winston, Alan and Khoo, Saye ORCID: 0000-0002-2769-0967
(2020) The development and validation of a novel LC-MS/MS method for the quantification of cenicriviroc in human plasma and cerebrospinal fluid. BIOMEDICAL CHROMATOGRAPHY, 34 (1). e4711-.

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Abstract

A high-performance liquid chromatography tandem mass spectrometric method was developed and validated for cenicriviroc (CVC) quantification in human plasma and cerebrospinal fluid (CSF). The method involved precipitation with acetonitrile and injecting supernatants onto the column. Separation was achieved on an XBridge C<sub>18</sub> column with a gradient elution of 0.1% formic acid in water and acetonitrile. Analyte detection was conducted in positive ion mode using selected reaction monitoring. The m/z transitions were: CVC (697.3 → 574.3) and CVC-d7 (704.4 → 574.3). Calibration curve ranged from 5 to 1000 ng/mL for plasma and from 0.241 to 15.0 ng/mL for CSF. The intra- and inter-day precision and accuracy were <15% for both plasma and CSF across four different concentrations. CVC recovery from plasma and artificial CSF was >90%. The method was utilized for the measurement of patients' plasma and CSF samples taking a dose of 50, 150 and 300 mg q.d.

Item Type: Article
Uncontrolled Keywords: cenicriviroc, LC-MS, MS, plasma, CSF, human serum albumin
Depositing User: Symplectic Admin
Date Deposited: 05 Nov 2019 09:50
Last Modified: 19 Jan 2023 00:20
DOI: 10.1002/bmc.4711
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3060640

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