Helassa, Nordine ORCID: 0000-0003-3743-1886, Nugues, Charlotte, Rajamanoharan, Dayani, Burgoyne, RD ORCID: 0000-0002-9219-0387 and Haynes, LP ORCID: 0000-0002-1296-0338
(2019)
A centrosome-localized calcium signal is essential for mammalian cell mitosis.
The FASEB Journal, 33 (12).
pp. 14602-14610.
Text
2019, Helassa et al, FASEB J.pdf - Published version Download (1MB) | Preview |
Abstract
Mitosis defects can lead to premature ageing and cancer. Understanding mitosis regulation therefore has important implications for human disease. Early data suggested that calcium (Ca2+) signals could influence mitosis, but these have hitherto not been observed in mammalian cells. Here, we reveal a prolonged yet spatially restricted Ca2+ signal at the centrosomes of actively dividing cells. Local buffering of the centrosomal Ca2+ signals, by flash photolysis of the caged Ca2+ chelator diazo‐2‐acetoxymethyl ester, arrests mitosis. We also provide evidence that this Ca2+ signal emanates from the endoplasmic reticulum. In summary, we characterize a unique centrosomal Ca2+ signal as a functionally essential input into mitosis.—Helassa, N., Nugues, C., Rajamanoharan, D., Burgoyne, R. D., Haynes, L. P. A centrosome‐localized calcium signal is essential for mammalian cell mitosis.
Item Type: | Article |
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Uncontrolled Keywords: | GCaMP, regulation, cell division |
Depositing User: | Symplectic Admin |
Date Deposited: | 07 Nov 2019 09:38 |
Last Modified: | 30 Jun 2023 14:03 |
DOI: | 10.1096/fj.201901662R |
Open Access URL: | https://www.fasebj.org/doi/10.1096/fj.201901662R |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3060749 |