Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected <i>Aedes aegypti</i>, <i>Aedes albopictus</i>, and <i>Culex</i> <i>quinquefasciatus</i> Mosquito Vectors

Hegde, Shivanand, Khanipov, Kamil, Albayrak, Levent, Golovko, George, Pimenova, Maria, Saldana, Miguel A, Rojas, Mark M, Hornett, Emily A ORCID: 0000-0002-7747-0583, Motl, Greg C, Fredregill, Chris L
et al (show 4 more authors) (2018) Microbiome Interaction Networks and Community Structure From Laboratory-Reared and Field-Collected <i>Aedes aegypti</i>, <i>Aedes albopictus</i>, and <i>Culex</i> <i>quinquefasciatus</i> Mosquito Vectors. FRONTIERS IN MICROBIOLOGY, 9 (SEP). 2160-.

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Microbial interactions are an underappreciated force in shaping insect microbiome communities. Although pairwise patterns of symbiont interactions have been identified, we have a poor understanding regarding the scale and the nature of co-occurrence and co-exclusion interactions within the microbiome. To characterize these patterns in mosquitoes, we sequenced the bacterial microbiome of <i>Aedes aegypti</i>, <i>Ae. albopictus</i>, and <i>Culex quinquefasciatus</i> caught in the field or reared in the laboratory and used these data to generate interaction networks. For collections, we used traps that attracted host-seeking or ovipositing female mosquitoes to determine how physiological state affects the microbiome under field conditions. Interestingly, we saw few differences in species richness or microbiome community structure in mosquitoes caught in either trap. Co-occurrence and co-exclusion analysis identified 116 pairwise interactions substantially increasing the list of bacterial interactions observed in mosquitoes. Networks generated from the microbiome of <i>Ae. aegypti</i> often included highly interconnected hub bacteria. There were several instances where co-occurring bacteria co-excluded a third taxa, suggesting the existence of tripartite relationships. Several associations were observed in multiple species or in field and laboratory-reared mosquitoes indicating these associations are robust and not influenced by environmental or host factors. To demonstrate that microbial interactions can influence colonization of the host, we administered symbionts to <i>Ae. aegypti</i> larvae that either possessed or lacked their resident microbiota. We found that the presence of resident microbiota can inhibit colonization of particular bacterial taxa. Our results highlight that microbial interactions in mosquitoes are complex and influence microbiome composition.

Item Type: Article
Uncontrolled Keywords: interaction networks, microbe-microbe interactions, gnotobiotic, gut symbiont, gut microbiome, Wolbachia
Depositing User: Symplectic Admin
Date Deposited: 28 Nov 2019 16:46
Last Modified: 17 Oct 2023 09:06
DOI: 10.3389/fmicb.2018.02160
Open Access URL: https://doi.org/10.3389/fmicb.2018.02160
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3063890