Saengchoowong, Suthat, Khongnomnan, Kritsada, Poomipak, Witthaya, Praianantathavorn, Kesmanee, Poovorawan, Yong, Zhang, Qibo and Payungporn, Sunchai
(2019)
High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses.
VIRUSES-BASEL, 11 (11).
E986-.
Abstract
Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin-Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigated in this cell line. Therefore, the present study aims at high-throughput analysis of canine microRNA profile upon infection of influenza B viruses. Briefly, MDCK cells were infected with Victoria or Yamagata lineage at MOI of 0.01. After being harvested at 6, 12 and 24 h post infection, microRNAs were subjected to high-throughput sequencing based on MiSeq platform (Illumina). The results demonstrated that five microRNAs including cfa-miR-197, cfa-miR-215, cfa-miR361, cfa-miR-1841, and cfa-miR-1842 were overexpressed in both Victoria and Yamagata lineage infections. Interestingly, computational prediction showed that karyopherin alpha 6 (KPNA6) was targeted by cfa-miR-197 and cfa-miR-215. Moreover, the binding sites of both microRNAs were assessed by 3'-UTR reporter assay. The results showed that only cfa-miR-197 could bind to the target sites of KPNA6, leading to suppressing luciferase activity. Additionally, silencing of KPNA6 was confirmed by overexpression of cfa-miR-197. This study provides canine microRNA responses to seasonal influenza B viruses, suggesting that virus-mediated microRNAs might play crucial roles in host gene regulation.
Item Type: | Article |
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Uncontrolled Keywords: | microRNAs, influenza B viruses, Yamagata, Victoria, MDCK, next-generation sequencing, Canis lupus familiaris |
Depositing User: | Symplectic Admin |
Date Deposited: | 29 Nov 2019 09:46 |
Last Modified: | 19 Jan 2023 00:18 |
DOI: | 10.3390/v11110986 |
Open Access URL: | https://doi.org/10.3390/v11110986 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3063990 |