High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses

Saengchoowong, Suthat, Khongnomnan, Kritsada, Poomipak, Witthaya, Praianantathavorn, Kesmanee, Poovorawan, Yong, Zhang, Qibo and Payungporn, Sunchai
(2019) High-Throughput MicroRNA Profiles of Permissive Madin-Darby Canine Kidney Cell Line Infected with Influenza B Viruses. VIRUSES-BASEL, 11 (11). E986-.

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Victoria and Yamagata lineages of influenza B viruses are globally circulating in seasonal epidemics. Madin-Darby canine kidney (MDCK) cells are permissive for viral isolation and vaccine manufacture. Nevertheless, the interplay between influenza B viruses and host microRNAs has not been investigated in this cell line. Therefore, the present study aims at high-throughput analysis of canine microRNA profile upon infection of influenza B viruses. Briefly, MDCK cells were infected with Victoria or Yamagata lineage at MOI of 0.01. After being harvested at 6, 12 and 24 h post infection, microRNAs were subjected to high-throughput sequencing based on MiSeq platform (Illumina). The results demonstrated that five microRNAs including cfa-miR-197, cfa-miR-215, cfa-miR361, cfa-miR-1841, and cfa-miR-1842 were overexpressed in both Victoria and Yamagata lineage infections. Interestingly, computational prediction showed that karyopherin alpha 6 (KPNA6) was targeted by cfa-miR-197 and cfa-miR-215. Moreover, the binding sites of both microRNAs were assessed by 3'-UTR reporter assay. The results showed that only cfa-miR-197 could bind to the target sites of KPNA6, leading to suppressing luciferase activity. Additionally, silencing of KPNA6 was confirmed by overexpression of cfa-miR-197. This study provides canine microRNA responses to seasonal influenza B viruses, suggesting that virus-mediated microRNAs might play crucial roles in host gene regulation.

Item Type: Article
Uncontrolled Keywords: microRNAs, influenza B viruses, Yamagata, Victoria, MDCK, next-generation sequencing, Canis lupus familiaris
Depositing User: Symplectic Admin
Date Deposited: 29 Nov 2019 09:46
Last Modified: 19 Jan 2023 00:18
DOI: 10.3390/v11110986
Open Access URL: https://doi.org/10.3390/v11110986
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3063990