Korf, Imke HE, Meier-Kolthoff, Jan P, Adriaenssens, Evelien M 
ORCID: 0000-0003-4826-5406, Kropinski, Andrew M, Nimtz, Manfred, Rohde, Manfred, van Raaij, Mark J and Wittmann, Johannes
(2019)
Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy.
VIRUSES-BASEL, 11 (5).
E454-.
ISSN 1999-4915, 1999-4915
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Still Something to Discover Novel Insights intoEscherichia coli Phage Diversity and Taxonomy.pdf - Published version Download (26MB) | Preview |
Abstract
The aim of this study was to gain further insight into the diversity of Escherichia coli phagesfollowed by enhanced work on taxonomic issues in that field. Therefore, we present the genomiccharacterization and taxonomic classification of 50 bacteriophages against <i>E. coli</i> isolated fromvarious sources, such as manure or sewage. All phages were examined for their host range on a setof different <i>E. coli</i> strains, originating, e.g., from human diagnostic laboratories or poultry farms.Transmission electron microscopy revealed a diversity of morphotypes (70% Myo-, 22% Sipho-, and8% Podoviruses), and genome sequencing resulted in genomes sizes from ~44 to ~370 kb.Annotation and comparison with databases showed similarities in particular to T4- and T5-likephages, but also to less-known groups. Though various phages against <i>E. coli</i> are already describedin literature and databases, we still isolated phages that showed no or only few similarities to otherphages, namely phages Goslar, PTXU04, and KWBSE43-6. Genome-based phylogeny andclassification of the newly isolated phages using VICTOR resulted in the proposal of new generaand led to an enhanced taxonomic classification of <i>E. coli</i> phages.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | bacteriophage, Escherichia coli, taxonomy, diversity, genomics |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 29 Nov 2019 13:13 |
| Last Modified: | 07 Dec 2024 08:55 |
| DOI: | 10.3390/v11050454 |
| Open Access URL: | https://doi.org/10.3390/v11050454 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3064051 |
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