Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy

Korf, Imke HE, Meier-Kolthoff, Jan P, Adriaenssens, Evelien M ORCID: 0000-0003-4826-5406, Kropinski, Andrew M, Nimtz, Manfred, Rohde, Manfred, van Raaij, Mark J and Wittmann, Johannes
(2019) Still Something to Discover: Novel Insights into Escherichia coli Phage Diversity and Taxonomy. VIRUSES-BASEL, 11 (5). E454-.

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The aim of this study was to gain further insight into the diversity of Escherichia coli phagesfollowed by enhanced work on taxonomic issues in that field. Therefore, we present the genomiccharacterization and taxonomic classification of 50 bacteriophages against <i>E. coli</i> isolated fromvarious sources, such as manure or sewage. All phages were examined for their host range on a setof different <i>E. coli</i> strains, originating, e.g., from human diagnostic laboratories or poultry farms.Transmission electron microscopy revealed a diversity of morphotypes (70% Myo-, 22% Sipho-, and8% Podoviruses), and genome sequencing resulted in genomes sizes from ~44 to ~370 kb.Annotation and comparison with databases showed similarities in particular to T4- and T5-likephages, but also to less-known groups. Though various phages against <i>E. coli</i> are already describedin literature and databases, we still isolated phages that showed no or only few similarities to otherphages, namely phages Goslar, PTXU04, and KWBSE43-6. Genome-based phylogeny andclassification of the newly isolated phages using VICTOR resulted in the proposal of new generaand led to an enhanced taxonomic classification of <i>E. coli</i> phages.

Item Type: Article
Uncontrolled Keywords: bacteriophage, Escherichia coli, taxonomy, diversity, genomics
Depositing User: Symplectic Admin
Date Deposited: 29 Nov 2019 13:13
Last Modified: 16 Oct 2023 09:31
DOI: 10.3390/v11050454
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