Mineral deposition promoted by resin-based sealants with different calcium phosphate additions.



Bohns, Fabio Rocha, Leitune, Vicente Castelo Branco, Balbinot, Gabriela de Souza, Samuel, Susana Maria Werner and Collares, Fabricio Mezzomo
(2019) Mineral deposition promoted by resin-based sealants with different calcium phosphate additions. Brazilian oral research, 33. e101 - e101.

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Abstract

The aim of this study was to evaluate the influence of different calcium phosphates (CaPs) on the physical, biological, and remineralizing properties of experimental resin-based sealants (RBSs). Triethylene-glycol dimethacrylate (90wt%) and bisphenol A-glycidyl methacrylate (10wt%) were used to produce resin-based sealants. Hydroxyapatite (SHAp), α-tricalcium phosphate (Sα-TCP) and octacalcium phosphate (SOCP) were added to the sealants in a 10wt% concentration. One group without CaPs was used as the control group (SCG). The degree of conversion (DC) was assessed with Fourier-transformed infrared spectroscopy, whereas cytotoxicity was tested with the HaCaT keratinocyte cell line. The ultimate tensile strength (UTS) was used to assess the mechanical strength of the experimental RBSs. Sealed enamel was used for colorimetric assay. Mineral deposition was assessed with Raman spectroscopy after 7, 14, and 28 days of sample immersion in artificial saliva. Scanning electron microscopy was used to analyze the surface morphology after 28 days of immersion. The addition of 10wt% of fillers significantly reduced the DC of sealants. SOCP groups showed reduced cell viability. Higher UTS was found for Sα-TCP and SHAp. The color analysis showed that SGC and demineralized teeth presented higher mismatches with the sound tissue. Mineral deposition was observed for SHAp and Sα-TCP after 7 days, with increased phosphate content and mineral deposits for SHAp after 28 days. RBS with the addition of 10% HAp promoted increased mineralization in vitro after 28 days, and did not affect cell viability, DC, mechanical properties, or RBS color in the enamel.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 11 Dec 2019 16:04
Last Modified: 18 Jun 2021 20:10
DOI: 10.1590/1807-3107bor-2019.vol33.0101
URI: https://livrepository.liverpool.ac.uk/id/eprint/3065918