Bnyan, Ruba, Khan, Iftikhar, Ehtezazi, Touraj, Saleem, Imran, Gordon, Sarah, O'Neill, Francis ORCID: 0000-0002-6009-1026 and Roberts, Matthew
(2019)
Formulation and optimisation of novel transfersomes for sustained release of local anaesthetic.
JOURNAL OF PHARMACY AND PHARMACOLOGY, 71 (10).
pp. 1508-1519.
Text
Final Accepted Manuscript 250719.pdf - Author Accepted Manuscript Download (1MB) | Preview |
Abstract
<h4>Objective</h4>To investigate the effect of formulation parameters on the preparation of transfersomes as sustained-release delivery systems for lidocaine and to develop and validate a new high-performance liquid chromatography (HPLC) method for analysis.<h4>Method</h4>Taguchi design of experiment (DOE) was used to optimise lidocaine-loaded transfersomes in terms of phospholipid, edge activator (EA) and phospholipid : EA ratio. Transfersomes were characterised for size, polydispersity index (PDI), charge and entrapment efficiency (%EE). A HPLC method for lidocaine quantification was optimised and validated using a mobile phase of 30%v/v PBS (0.01 m) : 70%v/v Acetonitrile at a flow rate of 1 ml/min, detected at 255 nm with retention time of 2.84 min. The release of lidocaine from selected samples was assessed in vitro.<h4>Key findings</h4>Transfersomes were 200 nm in size, with PDI ~ 0.3. HPLC method was valid for linearity (0.1-2 mg/ml, R<sup>2</sup> 0.9999), accuracy, intermediate precision and repeatability according to ICH guidelines. The %EE was between 44% and 56% and dependent on the formulation parameters. Taguchi DOE showed the effect of factors was in the rank order : lipid : EA ratio ˃ EA type ˃ lipid type. Optimised transfersomes sustained the release of lidocaine over 24 h.<h4>Conclusion</h4>Sustained-release, lidocaine-loaded transfersomes were successfully formulated and optimised using a DOE approach, and a new HPLC method for lidocaine analysis was developed and validated.
Item Type: | Article |
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Uncontrolled Keywords: | design of experiment, HPLC, local anaesthetic, sustained release, transfersomes |
Depositing User: | Symplectic Admin |
Date Deposited: | 23 Jan 2020 09:56 |
Last Modified: | 03 Mar 2023 07:40 |
DOI: | 10.1111/jphp.13149 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3071592 |