The detection sensitivity of commonly used singlet oxygen probes in aqueous environments


Entradas, Tiago, Waldron, Sarah and Volk, Martin ORCID: 0000-0003-3555-8584 (2020) The detection sensitivity of commonly used singlet oxygen probes in aqueous environments. Journal of Photochemistry and Photobiology B: Biology, 204. p. 111787.

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Abstract

The sensitivity for singlet oxygen (<sup>1</sup>O<sub>2</sub>) of two convenient <sup>1</sup>O<sub>2</sub> probes, 1,3-diphenylisobenzofuran (DPBF) and 9,10-Anthracenediyl-bis(methylene)dimalonic acid (ABDA), has been investigated in different aqueous environments. Both probes are commercially available at reasonable cost and can be used with standard UV-vis spectrometers. Although DPBF is not soluble in neat water and is not specific to the detection of <sup>1</sup>O<sub>2</sub>, it has very high, essentially diffusion-limited, reactivity towards <sup>1</sup>O<sub>2</sub>; it can trap up to 50% of all <sup>1</sup>O<sub>2</sub> created in alcohol/water or micellar solution, and even more when replacing H<sub>2</sub>O by D<sub>2</sub>O, which makes it highly useful when the process under investigation does not yield much <sup>1</sup>O<sub>2</sub>. On the other hand, ABDA has a much lower reactivity, reacting with only 2% of the singlet oxygen generated in H<sub>2</sub>O, as well as a smaller extinction coefficient, resulting in a much smaller spectroscopic response, but is soluble in neat water and is specific for <sup>1</sup>O<sub>2</sub>, allowing for discrimination from other reactive oxygen species. The results presented here not only allow a comparative assessment of the usefulness of the two <sup>1</sup>O<sub>2</sub> probes, but also provide a reference for an accurate absolute quantification of the amount of <sup>1</sup>O<sub>2</sub> generated in an experiment from the observed absorbance bleach.

Item Type: Article
Uncontrolled Keywords: Singlet oxygen, Singlet oxygen photogeneration, Singlet oxygen probe, DPBF, ABDA
Depositing User: Symplectic Admin
Date Deposited: 28 Jan 2020 11:19
Last Modified: 25 Jan 2024 09:49
DOI: 10.1016/j.jphotobiol.2020.111787
Open Access URL: https://doi.org/10.1016/j.jphotobiol.2020.111787
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3072317

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