Somerville, Tobi F, Corless, Caroline E, Neal, Timothy and Kaye, Stephen B 
ORCID: 0000-0003-0390-0592
(2018)
Effect of storage time and temperature on the detection of <i>Pseudomonas aeruginosa</i>, <i>Acanthamoeba</i> and Herpes Simplex Virus from corneal impression membranes.
JOURNAL OF MEDICAL MICROBIOLOGY, 67 (9).
pp. 1321-1325.
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Abstract
The effect of storage time and temperature on the recovery of pathogen DNA from polytetrafluorethylene (PTFE) was investigated. PTFE impression membranes were inoculated with Pseudomonas aeruginosa, Herpes Simplex Virus-1 (HSV-1) or Acanthamoeba and stored at -70 °C, -20 °C, +4 °C or +35 °C. PCR was performed on days 0, 1, 2, 3, 7 and months 1, 3 and 10 post-inoculation. We found no reduction in the DNA recovery of any of the studied microorganisms for the first 3 days of storage up to +35 °C. For HSV-1 and P. aeruginosa, storage for 3 months at +35 °C was associated with a significant reduction in DNA recovery (P<0.001), but not at +4 °C, -20 °C or -70 °C for 1 month for P. aeruginosa and for 10 months for HSV-1. Acanthamoeba DNA recovery was not affected by any storage parameters (P=0.203). These results will inform the investigation of microbial keratitis where access to microbiological testing is not readily available.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | microbial keratitis, corneal impression membrane, P. aeruginosa, Acanthamoeba, HSV-1 |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 06 Feb 2020 15:39 |
| Last Modified: | 14 Oct 2023 08:32 |
| DOI: | 10.1099/jmm.0.000812 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3073810 |
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