Use of a corneal impression membrane and PCR for the detection of herpes simplex virus type-1

Brunner, Matthias, Somerville, Tobi, Corless, Caroline E, Myneni, Jayavani, Rajhbeharrysingh, Tara, Tiew, Stephanie, Neal, Timothy and Kaye, Stephen B ORCID: 0000-0003-0390-0592
(2019) Use of a corneal impression membrane and PCR for the detection of herpes simplex virus type-1. JOURNAL OF MEDICAL MICROBIOLOGY, 68 (9). pp. 1324-1329.

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<b>Purpose</b>. To investigate the use of a corneal impression membrane (CIM) for the detection of herpes simplex virus type 1 (HSV-1) in suspected herpes simplex keratitis (HSK).<b>Methodology</b>. In the laboratory study, swabs and CIMs made from polytetrafluoroethylene were spiked with different concentrations of HSV-1. DNA was extracted and real-time PCR undertaken using two sets of primers. In the clinical study, consecutive patients presenting with suspected HSK were included. For each patient, samples were collected from corneal lesions with a swab and a CIM in random order. Clinical details were collected using a standardized clinical form and patients were categorized into probable, presumed and possible HSK.<b>Results</b>. There was no difference in the performance of both primer sets for all HSV-1 dilutions (<i>P</i>=0.83) using a CIM or between a CIM and a swab (<i>P</i>=0.18). In total, 110 patients were included. Overall, 73 patients (66.4 %) had probable, 20 patients (18.2 %) presumed and 17 patients (15.5 %) possible HSV-1 keratitis. The HSV-1 detection rate was significantly higher using a CIM (40/110, 36.4 %) than a swab (28/110, 25.5 %) (<i>P</i>=0.004). In the probable HSV keratitis group, the detection rate using a CIM was 43.8 % compared to 27.4 % for a swab (<i>P</i>=0.004). The cycle threshold values obtained for the conjunctival swabs were higher than those obtained for the CIMs (<i>P</i><0.001).<b>Conclusions</b>. In suspected HSK, a CIM is a useful alternative to a swab and more likely to detect the presence of HSV-1.

Item Type: Article
Uncontrolled Keywords: Herpes simplex keratitis, corneal impression membrane, detection of HSV-1 DNA, real time PCR
Depositing User: Symplectic Admin
Date Deposited: 07 Feb 2020 11:32
Last Modified: 19 Jan 2023 00:03
DOI: 10.1099/jmm.0.001041
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