Govaert, Marlies, Smet, Cindy, Walsh, James L ORCID: 0000-0002-6318-0892 and Van Impe, Jan FM
(2019)
Dual-Species Model Biofilm Consisting of <i>Listeria monocytogenes</i> and <i>Salmonella</i> Typhimurium: Development and Inactivation With Cold Atmospheric Plasma (CAP).
FRONTIERS IN MICROBIOLOGY, 10.
2524-.
Text
Dual-Species Model Biofilm Consisting of Listeria monocytogenes and Salmonella Typhimurium Development and Inactivation With.pdf - Published version Download (2MB) | Preview |
Abstract
Most environmental biofilms contain a variety of species. These species can establish cooperative and competitive interactions, possibly resulting in an increase or a decrease in antimicrobial resistance. Therefore, results obtained following inactivation of single-species biofilms by means of different technologies (e.g., Cold Atmospheric Plasma, CAP) should be validated for multi-species biofilms. First, a strongly adherent and mature <i>Listeria monocytogenes</i> and <i>S</i>. Typhimurium dual-species biofilm was developed by altering different incubation conditions, i.e., growth medium, incubation temperature, inoculum ratio of <i>L. monocytogenes</i> and <i>S</i>. Typhimurium cells, and incubation time. Adherence and maturity were quantified by means of optical density measurements and viable plate counts, respectively. Secondly, both the (1 day old) reference biofilm and a more mature 7 days old biofilm were treated for different CAP treatment times (0-30 min). Viable plate counts were again used to determine the (remaining) cell density. For both the biofilm development and inactivation, predictive models were applied to describe the growth/inactivation kinetics. Finally, the kinetics of the [1 and 7 day(s) old] dual-species biofilms were compared with those obtained for the corresponding single-species biofilms. Results implied that a strongly adherent and mature reference dual-species biofilm was obtained following 24 h of incubation at 25°C using 20-fold diluted TSB and an inoculum ratio of 1:1. Main observations regarding CAP inactivation were: (i) the dual-species biofilm age had no influence on the CAP efficacy, although a longer treatment time was required for the oldest biofilm, (ii) for the 1 day old biofilms, CAP treatment became less efficient for <i>S</i>. Typhimurium inactivation when this species was part of the dual-species biofilm, while <i>L. monocytogenes</i> inactivation was not influenced by the biofilm type, and (iii) for the 7 days old biofilms, CAP inactivation of both species became more efficient when they were part of the dual-species biofilms. It can be concluded that the efficacy of the CAP treatment is altered when cells become part of a dual-species biofilm, which is quite important with respect to a possible application of CAP for biofilm inactivation within the food industry.
Item Type: | Article |
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Uncontrolled Keywords: | cold atmospheric plasma (CAP), biofilms, inactivation, dual-species, single-species, L, monocytogenes, S, Typhimurium |
Depositing User: | Symplectic Admin |
Date Deposited: | 28 Feb 2020 12:22 |
Last Modified: | 14 Oct 2023 09:42 |
DOI: | 10.3389/fmicb.2019.02524 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3077048 |