Monitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry.



Sarsby, Joscelyn, McLean, Lynn, Harman, Victoria M ORCID: 0000-0002-0990-153X and Beynon, Robert J ORCID: 0000-0003-0857-495X
(2020) Monitoring recombinant protein expression in bacteria by rapid evaporative ionisation mass spectrometry. Rapid communications in mass spectrometry : RCM. e8670 - e8670.

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Abstract

RATIONALE:There is increasing interest in methods of direct analysis mass spectrometry that bypass complex sample preparation steps. METHODS:One of the most interesting new ionisation methods is rapid evaporative ionisation mass spectrometry (REIMS) in which samples are vapourised and the combustion products are subsequently ionised and analysed by mass spectrometry (Synapt G2si). The only sample preparation required is the recovery of a cell pellet from a culture that can be analysed immediately. RESULTS:We demonstrate that REIMS can be used to monitor the expression of heterologous recombinant proteins in Escherichia coli. Clear segregation was achievable between bacteria harvesting plasmids that were strongly expressed and other cultures in which the plasmid did not result in the expression of large amounts of recombinant product. CONCLUSIONS:REIMS has considerable potential as a near-instantaneous monitoring tool for protein production in a biotechnology environment.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 03 Mar 2020 09:13
Last Modified: 06 May 2021 06:10
DOI: 10.1002/rcm.8670
URI: https://livrepository.liverpool.ac.uk/id/eprint/3077473