The epigenetic landscape of oral squamous cell carcinoma

Jithesh, PV, Risk, JM ORCID: 0000-0002-8770-7783, Schache, AG ORCID: 0000-0001-9466-6038, Dhanda, J, Lane, B, Liloglou, T ORCID: 0000-0003-0460-1404 and Shaw, RJ ORCID: 0000-0001-7027-8997 (2013) The epigenetic landscape of oral squamous cell carcinoma. BRITISH JOURNAL OF CANCER, 108 (2). pp. 370-379. ISSN 0007-0920, 1532-1827

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Abstract

<h4>Background</h4>There is relatively little methylation array data available specifically for oral squamous cell carcinoma (OSCC). This study aims to compare the DNA methylome across a large cohort of tumour/normal pairs.<h4>Methods</h4>DNA was extracted from 44 OSCCs and paired normal mucosa. DNA methylation analysis employed the Illumina GoldenGate high-throughput array comprising 1505 CpG loci selected from 807 epigenetically regulated genes. This data was correlated with extracapsular spread (ECS), human papilloma virus (HPV) status, recurrence and 5-year survival.<h4>Results</h4>Differential methylation levels of a number of genes distinguished the tumour tissue sample from the matched normal. Putative methylation signatures for ECS and recurrence were identified. The concept of concordant methylation or CpG island methylator phenotype (CIMP) in OSCC is supported by our data, with an association between 'CIMP-high' and worse prognosis. Epigenetic deregulation of NOTCH4 signalling in OSCC was also observed, as part of a possible methylation signature for recurrence, with parallels to recently discovered NOTCH mutations in HNSCC. Differences in methylation in HPV-driven cases were seen, but are less significant than that has been recently proposed in other series.<h4>Conclusion</h4>Although OSCC seems as much an 'epigenetic' as a genetic disease, the translational potential of cancer epigenetics has yet to be fully exploited. This data points to the application of epigenetic biomarkers and targets available to further the development of therapy in OSCC.

Item Type:	Article
Additional Information:	## TULIP Type: Articles/Papers (Journal) ##
Uncontrolled Keywords:	oral squamous cell carcinoma, DNA methylation, microarray, prognosis
Depositing User:	Symplectic Admin
Date Deposited:	03 Mar 2020 08:51
Last Modified:	07 Dec 2024 14:05
DOI:	10.1038/bjc.2012.568
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3077553