A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study

Ashrafi, Mohammed, Xu, Yun ORCID: 0000-0003-3228-5111, Muhamadali, Howbeer, White, Iain, Wilkinson, Maxim, Hollywood, Katherine, Baguneid, Mohamed, Goodacre, Royston ORCID: 0000-0003-2230-645X and Bayat, Ardeshir (2020) A microbiome and metabolomic signature of phases of cutaneous healing identified by profiling sequential acute wounds of human skin: An exploratory study. PLOS ONE, 15 (2). e0229545-. ISSN 1932-6203, 1932-6203

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Official URL: https://doi.org/10.1371/journal.pone.0229545

Abstract

Profiling skin microbiome and metabolome has been utilised to gain further insight into wound healing processes. The aims of this multi-part temporal study in 11 volunteers were to analytically profile the dynamic wound tissue and headspace metabolome and sequence microbial communities in acute wound healing at days 0, 7, 14, 21 and 28, and to investigate their relationship to wound healing, using non-invasive quantitative devices. Metabolites were obtained using tissue extraction, sorbent and polydimethylsiloxane patches and analysed using GCMS. PCA of wound tissue metabolome clearly separated time points with 10 metabolites of 346 being involved in separation. Analysis of variance-simultaneous component analysis identified a statistical difference between the wound headspace metabolome, sites (P = 0.0024) and time points (P<0.0001), with 10 out of the 129 metabolites measured involved with this separation between sites and time points. A reciprocal relationship between Staphylococcus spp. and Propionibacterium spp. was observed at day 21 (P<0.05) with a statistical correlation between collagen and Propionibacterium (r = 0.417; P = 0.038) and Staphylococcus (r = -0.434; P = 0.03). Procrustes analysis showed a statistically significant similarity between wound headspace and tissue metabolome with non-invasive wound devices. This exploratory study demonstrates the temporal and dynamic nature of acute wound metabolome and microbiome presenting a novel class of biomarkers that correspond to wound healing, with further confirmatory studies now necessary.

Item Type:	Article
Uncontrolled Keywords:	Skin, Humans, Propionibacterium, Staphylococcus, Collagen, Wound Healing, Principal Component Analysis, Time Factors, Adult, Middle Aged, Female, Male, Metabolomics, Metabolome, Young Adult, Microbiota
Depositing User:	Symplectic Admin
Date Deposited:	10 Mar 2020 11:01
Last Modified:	07 Dec 2024 00:49
DOI:	10.1371/journal.pone.0229545
Open Access URL:	http://doi.org/10.1371/journal.pone.0229545
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3078460