Increased intracellular survival of Salmonella Typhimurium ST313 in HIV-1-infected primary human macrophages is not associated with Salmonella hijacking the HIV compartment.



Lê-Bury, G, Deschamps, C, Kizilyaprak, C, Blanchard, W, Daraspe, J, Dumas, A, Gordon, MA ORCID: 0000-0002-0629-0884, Hinton, JCD ORCID: 0000-0003-2671-6026, Humbel, BM and Niedergang, F
(2020) Increased intracellular survival of Salmonella Typhimurium ST313 in HIV-1-infected primary human macrophages is not associated with Salmonella hijacking the HIV compartment. Biol Cell.

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Abstract

BACKGROUND: Non-Typhoidal Salmonella (NTS) causes a severe invasive syndrome (iNTS disease) described in HIV-positive adults. The impact of HIV-1 on Salmonella pathogenesis and the molecular basis for the differences between these bacteria and classical diarrhoeal S. Typhimurium remains unclear. RESULTS: Here we show that iNTS-associated S. Typhimurium Sequence Type 313 (ST313) bacteria show greater intracellular survival in primary human macrophages, compared with a 'classical' diarrhoeal S. Typhimurium ST19 isolate. The increased intracellular survival phenotype of ST313 is more pronounced in HIV-infected macrophages. We explored the possibility that the bacteria take advantage of the HIV-associated viral-containing compartments created in human macrophages that have low pH. Confocal fluorescence microscopy and Focused Ion Beam-Scanning Electron Microscopy (FIB-SEM) tomography showed that Salmonella did not co-localise extensively with HIV-positive compartments. CONCLUSION: The capacity of ST313 bacteria to survive better than ST19 bacteria within primary human macrophages is enhanced in cells pre-infected with HIV-1. Our results indicate that the ST313 bacteria do not directly benefit from the niche created by the virus in HIV-1 infected macrophages, and that they might take advantage from a more globally modified host cell. SIGNIFICANCE: A better understanding of the interplay between HIV-1 and Salmonella is important not only for these bacteria but also for other opportunistic pathogens. This article is protected by copyright. All rights reserved.

Item Type: Article
Uncontrolled Keywords: FIB-SEM, human Immunodeficiency virus type 1 (HIV-1), invasive Salmonella enterica Typhimurium ST313, macrophages
Depositing User: Symplectic Admin
Date Deposited: 07 May 2020 10:31
Last Modified: 27 May 2021 21:10
DOI: 10.1111/boc.201900055
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3078683

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