Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project


Ahmed, Waqar M, Brinkman, Paul, Weda, Hans, Knobel, Hugo H, Xu, Yun ORCID: 0000-0003-3228-5111, Nijsen, Tamara M, Goodacre, Royston ORCID: 0000-0003-2230-645X, Rattray, Nicholas, Vink, Teunis J, Santonico, Marco et al (show 5 more authors) (2019) Methodological considerations for large-scale breath analysis studies: lessons from the U-BIOPRED severe asthma project. JOURNAL OF BREATH RESEARCH, 13 (1). 016001-.

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Abstract

Methods for breath sampling and analysis require robust quality assessment to minimise the risk of false discoveries. Planning large-scale multi-site breath metabolite profiling studies also requires careful consideration of systematic and random variation as a result of sampling and analysis techniques. In this study we use breath sample data from the recent U-BIOPRED cohort to evaluate and discuss some important methodological considerations such as batch variation and correction, variation between sites, storage and transportation, as well as inter-instrument analytical differences. Based on this we provide a summary of recommended best practices for new large scale multi-site studies.

Item Type: Article
Uncontrolled Keywords: multi-site studies, metabolomics, U-BIOPRED, quality assurance, breath analysis, volatile organic compounds, asthma
Depositing User: Symplectic Admin
Date Deposited: 23 Mar 2020 12:14
Last Modified: 18 Jan 2023 23:57
DOI: 10.1088/1752-7163/aae557
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3080191
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