Anderson, James 
ORCID: 0000-0003-0489-7997, Phelan, Marie, Foddy, Laura, Clegg, Peter and Peffers, Mandy ORCID: 0000-0001-6979-0440
(2020)
<i>Ex-Vivo</i>Equine Cartilage Explant Osteoarthritis Model - A Metabolomics and Proteomics Study.
BioRxiv.
2020.03.03.974501-.
ISSN 2692-8205
Abstract
Osteoarthritis (OA) is an age-related degenerative musculoskeletal disease characterised by loss of articular cartilage, synovitis, abnormal bone proliferation and subchondral bone sclerosis. Underlying OA pathogenesis is yet to be fully elucidated with no OA specific biomarkers in clinical use. Ex-vivo equine cartilage explants (n=5) were incubated in TNF-α/IL-1β supplemented culture media for 8 days, with media removed and replaced at 2, 5 and 8 days. Acetonitrile metabolite extractions of 8 day cartilage explants and media samples at all time points underwent 1D 1 H nuclear magnetic resonance metabolomic analysis with media samples also undergoing mass spectrometry proteomic analysis. Within the cartilage, metabolites glucose and lysine were elevated following TNF-α/IL-1β treatment whilst adenosine, alanine, betaine, creatine, myo-inositol and uridine levels decreased. Within the culture media, four, four and six differentially abundant metabolites and 154, 138 and 72 differentially abundant proteins, with > 2 fold change, were identified for 1-2 day, 3-5 day and 6-8 day time points respectively. Nine potential novel OA neopeptides were elevated in treated media. Our innovative study has identified differentially abundant metabolites, proteins and extracellular matrix derived neopeptides, providing insightful information on OA pathogenesis, enabling potential translation for clinical markers and possible new therapeutic targets.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 3401 Analytical Chemistry, 32 Biomedical and Clinical Sciences, 34 Chemical Sciences, 40 Engineering, 4003 Biomedical Engineering, 3205 Medical Biochemistry and Metabolomics, Osteoarthritis, Arthritis, Aging, Musculoskeletal |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 09 Apr 2020 10:23 |
| Last Modified: | 14 Mar 2025 00:32 |
| DOI: | 10.1101/2020.03.03.974501 |
| Open Access URL: | https://www.biorxiv.org/content/10.1101/2020.03.03... |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3082425 |
Altmetric
Altmetric