Al-Rasheed, Mohammed
(2020)
Immunopathogenesis of infectious bronchitis virus in chickens : the role of head-associated lymphoid and respiratory tissues
PhD thesis, University of Liverpool.
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Abstract
Infectious bronchitis virus (IBV) primarily replicates in the epithelial tissues of the respiratory tract, predominantly trachea. Little information is available on IBV replication and immune responses in head-associated lymphoid tissues (HALT) – Harderian Gland (HG) and choanal cleft, and respiratory (turbinate) tissues. To investigate the role of these tissues in comparison to trachea, 21-days of age commercial broiler chickens were challenged with IBV M41. Lachrymal fluid anti-IBV IgA levels were elevated at 4–5 days post-challenge (dpc). At 5 dpc, the viral load in the turbinate and choanal cleft was higher than those found in the HG, pharyngeal and tracheal tissues. Immunohistochemistry (IHC) confirmed presence of IBV replication in all examined tissues with peak viral antigen score at 3 dpc. There were high levels of TLR3, MDA5, IFN-α, IFN-β and IL-6 gene expression were found in the challenged compared to the unchallenged control group. Findings demonstrated an early innate immune response at 1–5 days after M41 challenge. More specifically, there was a marked up-regulation of TLR3, MDA5, IFN-β and IL-6 mRNA expression in the HG, choanal cleft, turbinate and trachea. With above findings and ... (continues)
| Item Type: | Thesis (PhD) |
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| Divisions: | Faculty of Health & Life Sciences |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 17 Aug 2020 15:25 |
| Last Modified: | 05 Nov 2024 20:49 |
| DOI: | 10.17638/03088421 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3088421 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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