A method for the permeabilization of live <i>Drosophila melanogaster</i> larvae to small molecules and cryoprotectants



Murray, Alex, Palmer, Daniel, Bennett, Daimark, Dwarampudi, Venkata and Pedro de Magalhaes, Joao
(2020) A method for the permeabilization of live <i>Drosophila melanogaster</i> larvae to small molecules and cryoprotectants. FLY, 14 (1-4). pp. 29-33.

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Abstract

The larvae of <i>Drosophila melanogaster</i> is a model organism widely used to study the muscular and nervous systems. <i>Drosophila</i> larvae are surrounded by a waxy cuticle that prevents permeation by most substances. Here we develop a method to remove this layer, rendering the larvae permeable to small molecules without causing death, allowing the larvae to develop to adulthood and reproduce. Permeability was assessed using fluorescein diacetate dye uptake, and mortality upon exposure to toxic levels of ethylene glycol (EG) and Dimethyl sulfoxide (DMSO). Potential uses for this method include drug delivery, toxicity assays, cryopreservation, staining, and fixation.

Item Type: Article
Uncontrolled Keywords: Drosophila, larvae, permeabilization, imaging, cryobiology, cryopreservation, method
Depositing User: Symplectic Admin
Date Deposited: 02 Jun 2020 08:03
Last Modified: 21 Mar 2024 09:58
DOI: 10.1080/19336934.2020.1724051
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3089270