Perylene Diimide Nanoprobes for In Vivo Tracking of Mesenchymal Stromal Cells Using Photoacoustic Imaging.



Yang, Yonghong, Fryer, Claudia, Sharkey, Jack ORCID: 0000-0002-0516-7622, Thomas, Aidan, Wais, Ulrike, Jackson, Alexander W, Wilm, Bettina ORCID: 0000-0002-9245-993X, Murray, Patricia and Zhang, Haifei ORCID: 0000-0001-5142-5824
(2020) Perylene Diimide Nanoprobes for In Vivo Tracking of Mesenchymal Stromal Cells Using Photoacoustic Imaging. ACS applied materials & interfaces, 12 (25). 27930 - 27939.

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Abstract

Noninvasive bioimaging techniques are critical for assessing the biodistribution of cellular therapies longitudinally. Among them, photoacoustic imaging (PAI) can generate high-resolution images with a tissue penetration depth of ∼4 cm. However, it is essential and still highly challenging to develop stable and efficient near-infrared (NIR) probes with low toxicity for PAI. We report here the preparation and use of perylene diimide derivative (PDI) with NIR absorbance (around 700 nm) as nanoprobes for tracking mesenchymal stromal cells (MSCs) in mice. Employing an in-house synthesized star hyperbranched polymer as a stabilizer is the key to the formation of stable PDI nanoparticles with low toxicity and high uptake by the MSCs. The PDI nanoparticles remain within the MSCs as demonstrated by in vitro and in vivo assessments. The PDI-labeled MSCs injected subcutaneously on the flanks of the mice are clearly visualized with PAI up to 11 days postadministration. Furthermore, bioluminescence imaging of PDI-labeled luciferase-expressing MSCs confirms that the administered cells remain viable for the duration of the experiment. These PDI nanoprobes thus have good potential for tracking administered cells in vivo using PAI.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 03 Jul 2020 09:08
Last Modified: 31 Jul 2021 07:53
DOI: 10.1021/acsami.0c03857
URI: https://livrepository.liverpool.ac.uk/id/eprint/3090668