Wu, Yunfei
(2020)
Characterization of the cross-interactions between Deformed Wing Virus (DWV), honey bee, and ectoparasitic mite, Tropilaelaps mercedesae
PhD thesis, University of Liverpool.
|
Text
200838998_Yunfei Wu_2020.pdf - Unspecified Download (5MB) | Preview |
Abstract
Recently, honey bee colony losses have been reported to be associated with both presence of the pathogen Deformed Wing Virus (DWV) and ectoparasitic mites. The DWV vectoring role of Varroa destructor is well established while the role of Tropilaelaps mercedesae in viral transmission has not been fully investigated. In this project, I examined the effects of both mite species infestation on honey bee by comparing the DWV copy number and alteration of DWV variants of individual pupae and their infesting mites. Infestation with either mite species causes increased DWV copy number in honey bee pupae, which proves the vector role of V. destructor on honey bee and as well as suggesting a similar viral vectoring role for T. mercedesae. Through artificial infestation and wound induction experiments, a biological and mechanical vector role for T. mercedesae has been established. I also identified a positive correlation between DWV copy number in pupae and copy number in infesting mites, which forms two clusters with either high or low copy number in both honey bee pupae and infesting mites. The same DWV type A variant was present in either low or high copy number in both honey bee pupae and infesting V. destructor ... (continues)
| Item Type: | Thesis (PhD) |
|---|---|
| Uncontrolled Keywords: | Honey bee, DWV, Tropilaelaps mercedesae, Varroa destructor, infection and replication, immunity |
| Divisions: | Faculty of Health & Life Sciences > Inst. Systems, Molec & Integrative Biology > Inst. Systems, Molec & Integrative Biology |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Aug 2020 09:04 |
| Last Modified: | 26 Jul 2025 15:53 |
| DOI: | 10.17638/03090688 |
| Supervisors: |
|
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3090688 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
Altmetric
Altmetric