Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus



Rose, Clair, Casas-Sanchez, Aitor ORCID: 0000-0001-5237-1223, Dyer, Naomi A, Solorzano, Carla, Beckett, Alison J ORCID: 0000-0001-8377-325X, Middlehurst, Ben, Marcello, Marco ORCID: 0000-0002-2392-8640, Haines, Lee R, Lisack, Jaime, Engstler, Markus
et al (show 3 more authors) (2020) Trypanosoma brucei colonizes the tsetse gut via an immature peritrophic matrix in the proventriculus. Nature Microbiology, 5 (7). 909 - 916.

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Abstract

The peritrophic matrix of blood-feeding insects is a chitinous structure that forms a protective barrier against oral pathogens and abrasive particles1. Tsetse flies transmit Trypanosoma brucei, which is the parasite that causes human sleeping sickness and is also partially responsible for animal trypanosomiasis in Sub-Saharan Africa. For this parasite to establish an infection in flies, it must first colonize the area between the peritrophic matrix and gut epithelium called the ectoperitrophic space. Although unproven, it is generally accepted that trypanosomes reach the ectoperitrophic space by penetrating the peritrophic matrix in the anterior midgut2,3,4. Here, we revisited this event using fluorescence- and electron-microscopy methodologies. We show that trypanosomes penetrate the ectoperitrophic space in which the newly made peritrophic matrix is synthesized by the proventriculus. Our model describes how these proventriculus-colonizing parasites can either migrate to the ectoperitrophic space or become trapped within peritrophic matrix layers to form cyst-like bodies that are passively pushed along the gut as the matrix gets remodelled. Furthermore, early proventricular colonization seems to be promoted by factors in trypanosome-infected blood that cause higher salivary gland infections and potentially increase parasite transmission.

Item Type: Article
Uncontrolled Keywords: cell migration, parasite development
Depositing User: Symplectic Admin
Date Deposited: 14 Jul 2020 09:45
Last Modified: 22 Jul 2021 04:10
DOI: 10.1038/s41564-020-0707-z
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3093550