Khoo, Jing Jing 
ORCID: 0000-0003-1285-6052, Kurtti, Timothy J, Husin, Nurul Aini, Beliavskaia, Alexandra ORCID: 0000-0002-1194-5689, Lim, Fang Shiang ORCID: 0000-0002-2650-7518, Zulkifli, Mulya Mustika Sari, Al-Khafaji, Alaa M, Hartley, Catherine, Darby, Alistair C ORCID: 0000-0002-3786-6209, Hughes, Grant L ORCID: 0000-0002-7567-7185 et al (show 3 more authors)
(2020)
Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain of <i>Wolbachia</i> in Tick Cell Lines.
Microorganisms, 8 (7).
E988-.
ISSN 2076-2607, 2076-2607
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Abstract
<i>Wolbachia</i> are intracellular endosymbionts of several invertebrate taxa, including insects and nematodes. Although <i>Wolbachia</i> DNA has been detected in ticks, its presence is generally associated with parasitism by insects. To determine whether or not <i>Wolbachia</i> can infect and grow in tick cells, cell lines from three tick species, <i>Ixodes scapularis</i>, <i>Ixodes ricinus</i> and <i>Rhipicephalus microplus</i>, were inoculated with <i>Wolbachia</i> strains <i>w</i>Stri and <i>w</i>AlbB isolated from mosquito cell lines. Homogenates prepared from fleas collected from cats in Malaysia were inoculated into an <i>I. scapularis</i> cell line. Bacterial growth and identity were monitored by microscopy and PCR amplification and sequencing of fragments of <i>Wolbachia</i> genes. The <i>w</i>Stri strain infected <i>Ixodes</i> spp. cells and was maintained through 29 passages. The <i>w</i>AlbB strain successfully infected <i>Ixodes</i> spp. and <i>R. microplus</i> cells and was maintained through 2-5 passages. A novel strain of <i>Wolbachia</i> belonging to the supergroup F, designated <i>w</i>CfeF, was isolated in <i>I. scapularis</i> cells from a pool of <i>Ctenocephalides</i> sp. cat fleas and maintained in vitro through two passages over nine months. This is the first confirmed isolation of a <i>Wolbachia</i> strain from a flea and the first isolation of any <i>Wolbachia</i> strain outside the "pandemic" A and B supergroups. The study demonstrates that tick cells can host multiple <i>Wolbachia</i> strains, and can be added to panels of insect cell lines to improve success rates in isolation of field strains of <i>Wolbachia</i>.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | Ctenocephalides, Malaysia, Wolbachia, flea, in vitro culture, phylogeny, tick cell line |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 22 Jul 2020 08:45 |
| Last Modified: | 12 Jun 2025 14:22 |
| DOI: | 10.3390/microorganisms8070988 |
| Open Access URL: | https://doi.org/10.3390/microorganisms8070988 |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3094788 |
Available Versions of this Item
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Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain of Wolbachia in Tick Cell Lines. (deposited 20 Jul 2020 07:42)
- Isolation and Propagation of Laboratory Strains and a Novel Flea-Derived Field Strain of <i>Wolbachia</i> in Tick Cell Lines. (deposited 22 Jul 2020 08:45) [Currently Displayed]
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