Mahbub, Moontaha, Hemm, Luisa, Yang, Yuxiao, Kaur, Ramanpreet, Carmen, Helder, Engl, Christoph, Huokko, Tuomas, Riediger, Matthias, Watanabe, Satoru, Liu, Luning 
ORCID: 0000-0002-8884-4819 et al (show 3 more authors)
(2020)
mRNA localisation, reaction centre biogenesis and thylakoid membrane targeting in cyanobacteria.
Nature Plants, 6 (9).
pp. 1179-1191.
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Abstract
The thylakoid membranes of cyanobacteria form a complex intracellular membrane system with a distinctive proteome. The sites of biogenesis of thylakoid proteins remain uncertain, as do the signals that direct thylakoid membrane-integral proteins to the thylakoids rather than to the plasma membrane. Here, we address these questions by using fluorescence in situ hybridization to probe the subcellular location of messenger RNA molecules encoding core subunits of the photosystems in two cyanobacterial species. These mRNAs cluster at thylakoid surfaces mainly adjacent to the central cytoplasm and the nucleoid, in contrast to mRNAs encoding proteins with other locations. Ribosome association influences the distribution of the photosynthetic mRNAs on the thylakoid surface, but thylakoid affinity is retained in the absence of ribosome association. However, thylakoid association is disrupted in a mutant lacking two mRNA-binding proteins, which probably play roles in targeting photosynthetic proteins to the thylakoid membrane.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | photosystem II, photosystem I, synechocystis |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 30 Jul 2020 15:35 |
| Last Modified: | 18 Jan 2023 23:38 |
| DOI: | 10.1038/s41477-020-00764-2 |
| Related URLs: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3095793 |
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