Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation



Rani, Ekta, Mohshim, Siti Akhtar, Yusof, Nor Hidayat, Ahmad, Muhammad Zamharir, Goodacre, Royston, Alang Ahmad, Shahrul Ainliah and Wong, Lu Shin
(2020) Sensitive and selective detection of DNA fragments associated with Ganoderma boninense by DNA-nanoparticle conjugate hybridisation. Journal of Materials Science, 55 (30). pp. 14965-14979.

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Abstract

<jats:sec> <jats:title>Abstract</jats:title> <jats:p>A colourimetric assay for the detection of DNA fragments associated with the oil palm pathogen <jats:italic>Ganoderma boninense</jats:italic> and other fungi DNA is reported. The assay is based on the aggregation of DNA-nanoparticle conjugates in the presence of complementary DNA from the target organism. Here, various designs of DNA-nanoparticle conjugates were evaluated, and it was found that the best design gave a visually observable colour change with as little as 2 pmol of double-stranded DNA analyte even in the presence of a large excess of a mixture of non-complementary DNA. Overall, this label-free system is rapid, sensitive, selective, simple in design, and easy to carry out. It does not require specialist equipment or specialist training for the interpretation of the results, and therefore has the potential to be deployed for agricultural diagnostics in the field.</jats:p> </jats:sec><jats:sec> <jats:title>Graphic abstract</jats:title> <jats:p> Development of a colourimetric assay based on DNA-nanoparticle conjugates for the oil palm pathogen <jats:italic>Ganoderma boninense</jats:italic>.</jats:p> </jats:sec>

Item Type: Article
Uncontrolled Keywords: 3401 Analytical Chemistry, 34 Chemical Sciences, Bioengineering, Nanotechnology, Genetics
Depositing User: Symplectic Admin
Date Deposited: 04 Aug 2020 09:25
Last Modified: 21 Jun 2024 10:22
DOI: 10.1007/s10853-020-05058-8
Open Access URL: https://link.springer.com/article/10.1007/s10853-0...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3096030