Antimicrobial Activity of Poly-epsilon-lysine Peptide Hydrogels Against <i>Pseudomonas aeruginosa</i>

Kennedy, Stephnie M, Deshpande, Pallavi ORCID: 0000-0001-9714-9917, Gallagher, Andrew G ORCID: 0000-0003-2448-4145, Horsburgh, Malcolm J ORCID: 0000-0002-3806-0039, Allison, Heather E ORCID: 0000-0003-0017-7992, Kaye, Stephen B ORCID: 0000-0003-0390-0592, Wellings, Donald A and Williams, Rachel L ORCID: 0000-0002-1954-0256
(2020) Antimicrobial Activity of Poly-epsilon-lysine Peptide Hydrogels Against <i>Pseudomonas aeruginosa</i>. INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE, 61 (10). 18-.

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<h4>Purpose</h4>To determine the antimicrobial activity of poly-epsilon-lysine (pɛK) functionalization of hydrogels against Pseudomonas aeruginosa.<h4>Methods</h4>Antimicrobial activities of pɛK and pɛK+ hydrogels were tested against both keratitis and a laboratory strain of Paeruginosa at a range of inocula sizes, over 4 and 24 hours. The number of viable CFU on pɛK and pɛK+ hydrogels or commercial contact lenses (CL) was investigated. Ex vivo porcine corneas were inoculated with Paeruginosa PAO1 (103 CFU) and incubated with pɛK+ hydrogels or commercial hydrogel CL for 24 hours and the effects of infection determined.<h4>Results</h4>PɛK+ hydrogels showed log reductions in viable CFU compared with pɛK hydrogels for all Paeruginosa strains, depending on inocula sizes and incubation time. After 24 hours pɛK+ hydrogels showed >5 and >7.5 log reduction in CFU compared with commercial hydrogel CL at 103 and 106 CFU, respectively. In an ex vivo porcine corneal infection model, pɛK+ hydrogels led to a significant decrease in viable PAO1 CFU and histologic analysis indicated a decreased infiltration of PAO1 into the stroma.<h4>Conclusions</h4>PɛK+ hydrogels demonstrated enhanced antimicrobial activity versus nonfunctionalized pɛK hydrogels against clinically relevant Paeruginosa strains. PɛK+ hydrogels have the potential to be used as a bandage CL with innate antimicrobial characteristics to minimize the risk of microbial keratitis.

Item Type: Article
Uncontrolled Keywords: hydrogel, pseudomonas keratitis, contact lenses, antimicrobial, bandage lens
Depositing User: Symplectic Admin
Date Deposited: 04 Aug 2020 08:38
Last Modified: 15 Mar 2024 02:28
DOI: 10.1167/iovs.61.10.18
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