Greaney Davies, Frances
(2020)
Defining the model for human papillomavirus driven oropharyngeal squamous cell carcinoma and the role of epithelial to mesenchymal transition in disease dissemination
PhD thesis, University of Liverpool.
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Abstract
Human papillomavirus (HPV)-positive oropharyngeal squamous cell carcinoma (OPSCC) incidence is rising rapidly in the western world. Understanding of this disease, and modelling the therapeutic response requires a suitable in vitro cell model. Groups around the world have struggled to culture OPSCC epithelial cells naturally infected with HPV and rely on a limited number of commercially available, long term cell cultures that do not match the common patient clinicopathological features of HPV-positive OPSCC. The research presented in this thesis sought to derive primary cells and low passage cell lines from HPV-positive OPSCC patients that retained HPV16 E6 and E7 oncogenes and activity. Although eight cell lines were successfully established, HPV16 viral oncogene expression (E6 and E7) were absent by real-time qPCR testing. If the available secondary cell lines are to be suitable representation of HPVpositive head and neck disease, a comprehensive understanding of these resources is of great importance. In this thesis, viral oncogene presence, expression, pathological HPV testing and migration profiling for the six HPV-positive head and neck cell lines confirmed that they remain suitable as an ongoing model ... (continues)
| Item Type: | Thesis (PhD) |
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| Divisions: | Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences > School of Medicine |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Jan 2021 11:22 |
| Last Modified: | 02 Nov 2024 12:36 |
| DOI: | 10.17638/03096687 |
| Supervisors: |
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| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3096687 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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