The utility of a differentiated preclinical liver model, HepaRG cells, in investigating delayed toxicity via inhibition of mitochondrial-replication induced by fialuridine.



Jolly, Carol E, Douglas, Oisin, Kamalian, Laleh, Jenkins, Rosalind E ORCID: 0000-0002-3730-1136, Beckett, Alison J ORCID: 0000-0001-8377-325X, Penman, Sophie L ORCID: 0000-0001-5326-1675, Williams, Dominic P ORCID: 0000-0002-0758-3152, Monshouwer, Mario, Simic, Damir, Snoeys, Jan
et al (show 2 more authors) (2020) The utility of a differentiated preclinical liver model, HepaRG cells, in investigating delayed toxicity via inhibition of mitochondrial-replication induced by fialuridine. Toxicology and applied pharmacology, 403. 115163 - ?.

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Abstract

During its clinical development fialuridine caused liver toxicity and the death of five patients. This case remains relevant due to the continued development of mechanistically-related compounds against a back-drop of simple in vitro models which remain limited for the preclinical detection of such delayed toxicity. Here, proteomic investigation of a differentiated, HepaRG, and proliferating, HepG2 cell model was utilised to confirm the presence of the hENT1 transporter, thymidine kinase-1 and -2 (TK1, TK2) and thymidylate kinase, all essential in order to reproduce the cellular activation and disposition of fialuridine in the clinic. Acute metabolic modification assays could only identify mitochondrial toxicity in HepaRG cells following extended dosing, 2 weeks. Toxic effects were observed around 10 μM, which is within a range of 10-15 X approximate Cmax. HepaRG cell death was accompanied by a significant decrease in mitochondrial DNA content, indicative of inhibition of mitochondrial replication, and a subsequent reduction in mitochondrial respiration and the activity of mitochondrial respiratory complexes, not replicated in HepG2 cells. The structural epimer of fialuridine, included as a pharmacological negative control, was shown to have no cytotoxic effects in HepaRG cells up to 4 weeks. Overall, these comparative studies demonstrate the HepaRG model has translational relevance for fialuridine toxicity and therefore may have potential in investigating the inhibition of mitochondrial replication over prolonged exposure for other toxicants.

Item Type: Article
Uncontrolled Keywords: Cell Line, Tumor, Mitochondria, Hepatocytes, Humans, DNA, Mitochondrial, Arabinofuranosyluracil, Antiviral Agents, DNA Replication, Dose-Response Relationship, Drug
Depositing User: Symplectic Admin
Date Deposited: 12 Aug 2020 14:09
Last Modified: 14 Jan 2021 09:11
DOI: 10.1016/j.taap.2020.115163
Open Access URL: https://doi.org/10.1016/j.taap.2020.115163
URI: https://livrepository.liverpool.ac.uk/id/eprint/3097310