Methods of inactivation of SARS-CoV-2 for downstream biological assays.



Patterson, Edward I, Prince, Tessa ORCID: 0000-0002-8796-2629, Anderson, Enyia R, Casas-Sanchez, Aitor ORCID: 0000-0001-5237-1223, Smith, Shirley L, Cansado-Utrilla, Cintia, Solomon, Tom ORCID: 0000-0001-7266-6547, Griffiths, Michael J, Acosta-Serrano, Álvaro, Turtle, Lance ORCID: 0000-0002-0778-1693
et al (show 1 more authors) (2020) Methods of inactivation of SARS-CoV-2 for downstream biological assays. The Journal of infectious diseases.

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Abstract

The scientific community has responded to the COVID-19 pandemic by rapidly undertaking research to find effective strategies to reduce the burden of this disease. Encouragingly, researchers from a diverse array of fields are collectively working towards this goal. Research with infectious SARS-CoV-2 is undertaken in high containment laboratories, however, it is often desirable to work with samples at lower containment levels. To facilitate the transfer of infectious samples from high containment laboratories, we have tested methods commonly used to inactivate virus and prepare the sample for additional experiments. Incubation at 80°C, a range of detergents, Trizol reagents and UV energies were successful at inactivating a high titre of SARS-CoV-2. Methanol and paraformaldehyde incubation of infected cells also inactivated the virus. These protocols can provide a framework for in house inactivation of SARS-CoV-2 in other laboratories, ensuring the safe use of samples in lower containment levels.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 28 Aug 2020 08:29
Last Modified: 23 Nov 2021 13:10
DOI: 10.1093/infdis/jiaa507
Open Access URL: https://academic.oup.com/jid/advance-article/doi/1...
URI: https://livrepository.liverpool.ac.uk/id/eprint/3098834