Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii

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Xu, Nannan, Oltmanns, Anne, Zhao, Longsheng, Girot, Antoine, Karimi, Marzieh, Hoepfner, Lara, Kelterborn, Simon, Scholz, Martin, Beissel, Julia, Hegemann, Peter et al (show 4 more authors) , Baeumchen, Oliver, Liu, Lu-Ning ORCID: 0000-0002-8884-4819, Huang, Kaiyao and Hippler, Michael (2020) Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii. ELIFE, 9. e58805-.

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Abstract

For the unicellular alga Chlamydomonas reinhardtii, the presence of N-glycosylated proteins on the surface of two flagella is crucial for both cell-cell interaction during mating and flagellar surface adhesion. However, it is not known whether only the presence or also the composition of N-glycans attached to respective proteins is important for these processes. To this end, we tested several C. reinhardtii insertional mutants and a CRISPR/Cas9 knockout mutant of xylosyltransferase 1A, all possessing altered N-glycan compositions. Taking advantage of atomic force microscopy and micropipette force measurements, our data revealed that reduction in N-glycan complexity impedes the adhesion force required for binding the flagella to surfaces. This results in impaired polystyrene bead binding and transport but not gliding of cells on solid surfaces. Notably, assembly, intraflagellar transport, and protein import into flagella are not affected by altered N-glycosylation. Thus, we conclude that proper N-glycosylation of flagellar proteins is crucial for adhering C. reinhardtii cells onto surfaces, indicating that N-glycans mediate surface adhesion via direct surface contact.

Item Type:	Article
Uncontrolled Keywords:	Flagella, Chlamydomonas reinhardtii, Polysaccharides, Microscopy, Atomic Force, Cell Adhesion, Glycosylation, Gene Knockout Techniques, CRISPR-Cas Systems, Gene Editing, CRISPR-Associated Protein 9
Depositing User:	Symplectic Admin
Date Deposited:	11 Dec 2020 09:20
Last Modified:	18 Jan 2023 23:18
DOI:	10.7554/eLife.58805
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3109942

Available Versions of this Item

• Altered N-glycan composition impacts flagella mediated adhesion in Chlamydomonas reinhardtii. (deposited 10 Dec 2020 08:26)
  • Altered N-glycan composition impacts flagella-mediated adhesion in Chlamydomonas reinhardtii. (deposited 11 Dec 2020 09:20) [Currently Displayed]