Swainsbury, David JK, Qian, Pu, Jackson, Philip J, Faries, Kaitlyn M, Niedzwiedzki, Dariusz M, Martin, Elizabeth C, Farmer, David A, Malone, Lorna A, Thompson, Rebecca F, Ranson, Neil A et al (show 6 more authors)
(2021)
Structures of Rhodopseudomonas palustris RC-LH1 complexes with open or closed quinone channels
SCIENCE ADVANCES, 7 (3).
eabe2631-.
ISSN 2375-2548, 2375-2548
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Text
Swainsbury et al, 2021 Rps palustris RC-LH1 Structures.pdf - Published version Download (2MB) | Preview |
Abstract
The reaction-center light-harvesting complex 1 (RC-LH1) is the core photosynthetic component in purple phototrophic bacteria. We present two cryo-electron microscopy structures of RC-LH1 complexes from Rhodopseudomonas palustris. A 2.65-Å resolution structure of the RC-LH114-W complex consists of an open 14-subunit LH1 ring surrounding the RC interrupted by protein-W, whereas the complex without protein-W at 2.80-Å resolution comprises an RC completely encircled by a closed, 16-subunit LH1 ring. Comparison of these structures provides insights into quinone dynamics within RC-LH1 complexes, including a previously unidentified conformational change upon quinone binding at the RC QB site, and the locations of accessory quinone binding sites that aid their delivery to the RC. The structurally unique protein-W prevents LH1 ring closure, creating a channel for accelerated quinone/quinol exchange.
| Item Type: | Article |
|---|---|
| Uncontrolled Keywords: | 34 Chemical Sciences, 3406 Physical Chemistry, 1.1 Normal biological development and functioning, Generic health relevance |
| Depositing User: | Symplectic Admin |
| Date Deposited: | 14 Jan 2021 10:23 |
| Last Modified: | 16 Jun 2026 15:32 |
| DOI: | 10.1126/sciadv.abe2631 |
| Open Access URL: | https://advances.sciencemag.org/content/7/3/eabe26... |
| Related Websites: | |
| URI: | https://livrepository.liverpool.ac.uk/id/eprint/3113607 |
| Disclaimer: | The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate. |
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