Minimal inhibitory concentration of seven antimicrobials to Mycoplasma gallisepticum and Mycoplasma synoviae isolates from six European countries

de Jong, Anno, Youala, Myriam, Klein, Ulrich, El Garch, Farid, Simjee, Shabbir, Moyaert, Hilde, Rose, Markus, Gautier-Bouchardon, Anne V, Catania, Salvatore, Ganapathy, Kannan ORCID: 0000-0002-9401-3560
et al (show 4 more authors) (2021) Minimal inhibitory concentration of seven antimicrobials to Mycoplasma gallisepticum and Mycoplasma synoviae isolates from six European countries. AVIAN PATHOLOGY, 50 (2). pp. 161-173.

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<i>Mycoplasma gallisepticum</i> and <i>Mycoplasma synoviae</i> are bacterial pathogens that cause disease in poultry, adversely affecting their health and welfare, and are a financial burden on producers. This manuscript describes the results of the MycoPath project that is the first international antimicrobial susceptibility programme for mycoplasma pathogens isolated from poultry. Improved comparative analysis of minimal inhibitory concentration (MIC) results from participating countries was facilitated by using one laboratory determining all MICs. Chicken and turkey isolates were obtained from France, Germany, Great Britain, Hungary, Italy and Spain during 2014-2016. One isolate per farm was retained. The MIC of seven antimicrobial agents was determined using a broth microdilution method, with Friis Medium (<i>M. gallisepticum</i>) or Modified Chanock's Medium (<i>M. synoviae</i>). Of the 222 isolates recovered, 82 were <i>M. gallisepticum</i> and 130 were <i>M. synoviae. M. gallisepticum</i> MIC<sub>50/90</sub> values were 0.12/0.5, 2/8, 0.5/4, 0.12/>64, 0.008/0.062, 0.008/32, 0.062/4 mg/l for doxycycline, enrofloxacin, oxytetracycline, spiramycin, tiamulin, tilmicosin and tylosin, respectively. For <i>M. synoviae</i>, the values were 0.5/1, 8/16, 0.5/1, 0.5/8, 0.25/0.5, 0.062/2 and 0.062/16 mg/l respectively<i>.</i> A bimodal MIC distribution for the fluoroquinolone (enrofloxacin) and the macrolides (spiramycin, tilmicosin and tylosin) indicate that both species have sub-populations that are less susceptible <i>in vitro</i> to those antimicrobials. Some differences in susceptibilities were observed according to host species, <i>Mycoplasma</i> species, and country of origin. This study provides a baseline of novel data for future monitoring of antimicrobial resistance in poultry <i>Mycoplasma</i> species. Additionally, this information will facilitate the selection of the antimicrobial agents most likely to be effective, thus ensuring their minimal use with targeted and correct therapeutic treatments.<b>Highlights</b> First large-scale pan-European collection of representative <i>Mg</i> and <i>Ms</i> isolates.MIC values assessed in central laboratory for <i>Mg</i> and <i>Ms</i> from chickens and turkeys.Range of MIC values for 82 <i>Mg</i> and 130 <i>Ms</i> isolates to seven licenced antibiotics shown.Data can be used to help determine <i>Mg</i> and <i>Ms</i> veterinary-specific breakpoints.

Item Type: Article
Uncontrolled Keywords: Antimicrobial resistance, broth microdilution, chicken, MIC, Mycoplasma gallisepticum, Mycoplasma synoviae, turkey
Depositing User: Symplectic Admin
Date Deposited: 19 Feb 2021 14:31
Last Modified: 17 Apr 2023 13:07
DOI: 10.1080/03079457.2020.1861216
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