Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing bloodstream infection in Malawi



Rodwell, Ella V ORCID: 0000-0002-6358-6542, Wenner, Nicolas, Pulford, Caisey V ORCID: 0000-0003-2500-9119, Cai, Yueyi, Bowers-Barnard, Arthur, Beckett, Alison, Rigby, Jonathan ORCID: 0000-0001-5143-0981, Picton, David M ORCID: 0000-0002-8456-0237, Blower, Tim R ORCID: 0000-0002-7390-6458, Feasey, Nicholas A ORCID: 0000-0003-4041-1405
et al (show 2 more authors) Isolation and characterisation of bacteriophages with activity against invasive non-typhoidal Salmonella causing bloodstream infection in Malawi.

WarningThere is a more recent version of this item available.
Access the full-text of this item by clicking on the Open Access link.

Abstract

<jats:label>1</jats:label><jats:title>Abstract</jats:title><jats:p>In recent years, novel lineages of invasive non-typhoidal <jats:italic>Salmonella</jats:italic> (iNTS) serovars Typhimurium and Enteritidis have been identified in patients with bloodstream infection in sub-Saharan Africa. Here, we isolated and characterised 32 phages capable of infecting <jats:italic>S</jats:italic>. Typhimurium and <jats:italic>S</jats:italic>. Enteritidis, from water sources in Malawi and the UK. The phages were classified in three major phylogenetic clusters that were geographically distributed. In terms of host range, Cluster 1 phages were able to infect all bacterial hosts tested, whereas Clusters 2 and 3 had a more restricted profile. Cluster 3 contained two sub-clusters, and 3.b contained the most novel isolates. This study represents the first exploration of the potential for phages to target the lineages of <jats:italic>Salmonella</jats:italic> that are responsible for bloodstream infections in sub-Saharan Africa.</jats:p>

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 22 Feb 2021 16:23
Last Modified: 16 Mar 2021 09:10
DOI: 10.1101/2021.02.18.431836
Open Access URL: https://doi.org/10.1101/2021.02.18.431836
URI: https://livrepository.liverpool.ac.uk/id/eprint/3115960

Available Versions of this Item