Helical ensembles out-perform ideal helices in Molecular Replacement



Rodríguez, Filomeno Sánchez, Simpkin, Adam ORCID: 0000-0003-1883-9376, Davies, Owen ORCID: 0000-0002-3806-5403, Keegan, Ronan and Rigden, Daniel
(2020) Helical ensembles out-perform ideal helices in Molecular Replacement.

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Abstract

The conventional approach in molecular replacement (MR) is the use of a related structure as a search model. However, this is not always possible as the availability of such structures can be scarce for poorly characterised families of proteins. In these cases, alternative approaches can be explored, such as the use of small ideal fragments that share high albeit local structural similarity with the unknown protein. Earlier versions of AMPLE enabled the trialling of a library of ideal helices, which worked well for largely helical proteins at suitable resolution. Here we explore the performance of libraries of helical ensembles created by clustering helical segments. The impacts of different B-factor treatments and different degrees of structural heterogeneity are explored. We observed a 30% increase in the number of solutions obtained by AMPLE when using this new set of ensembles compared to performance with ideal helices. The boost of performance was notable across three different folds: transmembrane, globular and coiled-coil structures. Furthermore, the increased effectiveness of these ensembles was coupled to a reduction of the time required by AMPLE to reach a solution. AMPLE users can now take full advantage of this new library of search models by activating the “helical ensembles” mode.

Item Type: Article
Depositing User: Symplectic Admin
Date Deposited: 24 Feb 2021 16:10
Last Modified: 09 Sep 2021 13:25
DOI: 10.1101/2020.06.16.154690
Open Access URL: https://scripts.iucr.org/cgi-bin/paper?S2059798320...
URI: https://livrepository.liverpool.ac.uk/id/eprint/3115990