Material Characterisation and Stratification of Conjunctival Epithelial Cells on Electrospun Poly(ε-Caprolactone) Fibres Loaded with Decellularised Tissue Matrices.



Bosworth, Lucy A ORCID: 0000-0002-6726-4663, Doherty, Kyle G ORCID: 0000-0002-7616-1295, Hsuan, James D, Cray, Samuel P, D'Sa, Raechelle A ORCID: 0000-0003-2651-8783, Pineda Molina, Catalina, Badylak, Stephen F and Williams, Rachel L ORCID: 0000-0002-1954-0256
(2021) Material Characterisation and Stratification of Conjunctival Epithelial Cells on Electrospun Poly(ε-Caprolactone) Fibres Loaded with Decellularised Tissue Matrices. Pharmaceutics, 13 (3). 318-.

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Abstract

The conjunctiva, an under-researched yet incredibly important tissue, plays key roles in providing protection to the eye and maintaining homeostasis of its ocular surface. Multiple diseases can impair conjunctival function leading to severe consequences that require surgical intervention. Small conjunctival defects can be repaired relatively easily, but larger defects rely on tissue grafts which generally do not provide adequate healing. A tissue engineering approach involving a biomaterial substrate capable of supporting a stratified epithelium with embedded, mucin-secreting goblet cells offers a potential solution. As a first step, this study aimed to induce stratification of human conjunctival epithelial cells cultured on electrospun scaffolds composed from poly(ε-caprolactone) (PCL) and decellularised tissue matrix (small intestinal submucosa (SIS) or urinary bladder matrix (UBM)) and held at the air/liquid interface. Stratification, up to 5 cell layers, occurred more frequently on scaffolds containing PCL + UBM. Incorporation of these decellularised tissue matrices also impacted material properties, with significant changes occurring to their fibre diameter, tensile properties, and chemical composition throughout the scaffold structure compared to PCL alone. These matrix containing scaffolds warrant further long-term investigation as a potential advanced therapy medicinal product for conjunctiva repair and regeneration.

Item Type: Article
Uncontrolled Keywords: electrospinning, conjunctiva, decellularized tissue matrix, small intestinal submucosa, urinary bladder matrix, polycaprolactone, fiber, tissue engineering, stratification, conjunctival epithelial cells
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User: Symplectic Admin
Date Deposited: 15 Mar 2021 11:41
Last Modified: 06 Mar 2023 19:57
DOI: 10.3390/pharmaceutics13030318
Open Access URL: http://doi.org/10.3390/pharmaceutics13030318
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3117355