Identification of Reduced Host Transcriptomic Signatures for Tuberculosis Disease and Digital PCR-Based Validation and Quantification


Gliddon, Harriet D, Kaforou, Myrsini, Alikian, Mary, Habgood-Coote, Dominic, Zhou, Chenxi, Oni, Tolu, Anderson, Suzanne T, Brent, Andrew J, Crampin, Amelia C, Eley, Brian et al (show 11 more authors) (2021) Identification of Reduced Host Transcriptomic Signatures for Tuberculosis Disease and Digital PCR-Based Validation and Quantification. Frontiers in Immunology, 12. 637164-. ISSN 1664-3224, 1664-3224

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Abstract

<jats:p>Recently, host whole blood gene expression signatures have been identified for diagnosis of tuberculosis (TB). Absolute quantification of the concentrations of signature transcripts in blood have not been reported, but would facilitate diagnostic test development. To identify minimal transcript signatures, we applied a transcript selection procedure to microarray data from African adults comprising 536 patients with TB, other diseases (OD) and latent TB (LTBI), divided into training and test sets. Signatures were further investigated using reverse transcriptase (RT)—digital PCR (dPCR). A four-transcript signature (<jats:italic>GBP6, TMCC1, PRDM1</jats:italic>, and <jats:italic>ARG1</jats:italic>) measured using RT-dPCR distinguished TB patients from those with OD (area under the curve (AUC) 93.8% (CI<jats:sub>95%</jats:sub> 82.2–100%). A three-transcript signature (<jats:italic>FCGR1A, ZNF296, and C1QB</jats:italic>) differentiated TB from LTBI (AUC 97.3%, CI<jats:sub>95%</jats:sub>: 93.3–100%), regardless of HIV. These signatures have been validated across platforms and across samples offering strong, quantitative support for their use as diagnostic biomarkers for TB.</jats:p>

Item Type: Article
Uncontrolled Keywords: tuberculosis, transcriptomics, dPCR, gene expression, signatures, biomarkers
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 24 Mar 2021 10:38
Last Modified: 07 Dec 2024 21:41
DOI: 10.3389/fimmu.2021.637164
Open Access URL: https://www.frontiersin.org/articles/10.3389/fimmu...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3118126
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