A haemagglutination test for rapid detection of antibodies to SARS-CoV-2

Collapse authors list.
Townsend, Alain, Rijal, Pramila, Xiao, Julie, Tan, Tiong Kit, Huang, Kuan-Ying A, Schimanski, Lisa, Huo, Jiandong, Gupta, Nimesh, Rahikainen, Rolle, Matthews, Philippa C et al (show 53 more authors) , Crook, Derrick, Hoosdally, Sarah, Dunachie, Susanna, Barnes, Eleanor, Street, Teresa, Conlon, Christopher P, Frater, John, Arancibia-Carcamo, Carolina V, Rudkin, Justine, Stoesser, Nicole, Karpe, Fredrik, Neville, Matthew, Ploeg, Rutger, Oliveira, Marta, Roberts, David J, Lamikanra, Abigail A, Tsang, Hoi Pat, Bown, Abbie, Vipond, Richard, Mentzer, Alexander J, Knight, Julian C, Kwok, Andrew J, Screaton, Gavin R, Mongkolsapaya, Juthathip, Dejnirattisai, Wanwisa, Supasa, Piyada, Klenerman, Paul, Dold, Christina, Baillie, J Kenneth, Moore, Shona C ORCID: 0000-0001-8610-2806, Openshaw, Peter JM, Semple, Malcolm G ORCID: 0000-0001-9700-0418, Turtle, Lance CW ORCID: 0000-0002-0778-1693, Ainsworth, Mark, Allcock, Alice, Beer, Sally, Bibi, Sagida, Skelly, Donal, Stafford, Lizzy, Jeffrey, Katie, O'Donnell, Denise, Clutterbuck, Elizabeth, Espinosa, Alexis, Mendoza, Maria, Georgiou, Dominique, Lockett, Teresa, Martinez, Jose, Perez, Elena, Sanchez, Veronica Gallardo, Scozzafava, Giuseppe, Sobrinodiaz, Alberto, Thraves, Hannah and Joly, Etienne (2021) A haemagglutination test for rapid detection of antibodies to SARS-CoV-2 NATURE COMMUNICATIONS, 12 (1). 1951-. ISSN 2041-1723, 2041-1723

Access the full-text of this item by clicking on the Open Access link.

Abstract

Serological detection of antibodies to SARS-CoV-2 is essential for establishing rates of seroconversion in populations, and for seeking evidence for a level of antibody that may be protective against COVID-19 disease. Several high-performance commercial tests have been described, but these require centralised laboratory facilities that are comparatively expensive, and therefore not available universally. Red cell agglutination tests do not require special equipment, are read by eye, have short development times, low cost and can be applied at the Point of Care. Here we describe a quantitative Haemagglutination test (HAT) for the detection of antibodies to the receptor binding domain of the SARS-CoV-2 spike protein. The HAT has a sensitivity of 90% and specificity of 99% for detection of antibodies after a PCR diagnosed infection. We will supply aliquots of the test reagent sufficient for ten thousand test wells free of charge to qualified research groups anywhere in the world.

Item Type:	Article
Uncontrolled Keywords:	Humans, Antibodies, Monoclonal, Antibodies, Viral, Enzyme-Linked Immunosorbent Assay, Agglutination Tests, Hemagglutination Tests, Sensitivity and Specificity, Polymerase Chain Reaction, Point-of-Care Systems, Spike Glycoprotein, Coronavirus, Seroconversion, COVID-19, SARS-CoV-2, COVID-19 Testing
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Infection, Vet & Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	09 Apr 2021 08:16
Last Modified:	16 Jun 2026 09:19
DOI:	10.1038/s41467-021-22045-y
Open Access URL:	https://www.nature.com/articles/s41467-021-22045-y
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3118758
Disclaimer:	The University of Liverpool is not responsible for content contained on other websites from links within repository metadata. Please contact us if you notice anything that appears incorrect or inappropriate.