Insertion mutation in Tnfrsf11a causes a Paget's disease‐like phenotype in heterozygous mice and osteopetrosis in homozygous mice



Alonso, Nerea, Wani, Sachin, Rose, Lorraine, van't Hof, Rob J, Ralston, Stuart H and Albagha, Omar ME
(2021) Insertion mutation in Tnfrsf11a causes a Paget's disease‐like phenotype in heterozygous mice and osteopetrosis in homozygous mice. Journal of Bone and Mineral Research, 36 (7). pp. 1376-1386.

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Abstract

Early onset familial Paget's disease of bone (EoPDB), familial expansile osteolysis, and expansile skeletal hyperphosphatasia are related disorders caused by insertion mutations in exon 1 of the TNFRSF11A gene, which encodes receptor activator of nuclear factor κB (RANK) protein. To understand the mechanisms underlying these disorders, we developed a mouse model carrying the 75dup27 mutation which causes EoPDB. Mice heterozygous for the mutation (Tnfrsf11a<sup>75dup27/-</sup> ) developed a PDB-like disorder with focal osteolytic lesions in the hind limbs with increasing age. Treatment of these mice with zoledronic acid completely prevented the development of lesions. Studies in vitro showed that RANK ligand (RANKL)-induced osteoclast formation and signaling was impaired in bone marrow cells from Tnfrsf11a<sup>75dup27/-</sup> animals, but that osteoclast survival was increased independent of RANKL stimulation. Surprisingly, Tnfrsf11a<sup>75dup27/75dup27</sup> homozygotes had osteopetrosis at birth, with complete absence of osteoclasts. Bone marrow cells from these mice failed to form osteoclasts in response to RANKL and macrophage colony-stimulating factor (M-CSF) stimulation. This intriguing study has shown that in heterozygous form, the 75dup27 mutation causes focal osteolytic lesions in vivo reminiscent of the human disorder and extends osteoclast survival independently of RANKL signaling. In homozygous form, however, the mutation causes osteopetrosis due to failure of osteoclast formation and insensitivity to RANKL stimulation. © 2021 The Authors. Journal of Bone and Mineral Research published by Wiley Periodicals LLC on behalf of American Society for Bone and Mineral Research (ASBMR)..

Item Type: Article
Uncontrolled Keywords: 75dup27, MOUSE MODEL, OSTEOCLAST, PAGET&apos, S DISEASE OF BONE, RANK
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences
Depositing User: Symplectic Admin
Date Deposited: 13 Apr 2021 07:27
Last Modified: 15 Mar 2024 04:09
DOI: 10.1002/jbmr.4288
Open Access URL: https://asbmr.onlinelibrary.wiley.com/doi/abs/10.1...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3119098