Kobpornchai, Porntida, Tiffney, Ellen-Alana, Adisakwattana, Poom and Flynn, Robin J ORCID: 0000-0001-5304-3088
(2021)
<i>Trichinella spiralis</i> cystatin, TsCstN, modulates STAT4/IL-12 to specifically suppress IFN-γ production.
CELLULAR IMMUNOLOGY, 362.
104303-.
Abstract
We have previously identified a cystatin, TsCstN, derived from the L1 stage of Trichinella spiralis and have shown that this protein is internalised in macrophages. Here we sought to address if this macrophage-TsCstN interaction could alter downstream T-cell priming. Using LPS-primed macrophages to stimulate T-cells in a co-culture system with or without TsCstN we assessed the resultant T-cell outcomes. IFN-γ, both protein and mRNA, but not IL-17A was negatively regulated by inclusion of TsCstN during macrophage priming. We identified a cell-cell contact independent change in the levels of IL-12 that led to altered phosphorylated STAT4 levels and translocation. TsCstN also negatively regulated the autonomous response in the myotubule cell line, C2C12. This work identifies a potential pathyway for L1 larvae to evade protective Th1 based immune responses and establish muscle-stage T. spiralis infection.
Item Type: | Article |
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Uncontrolled Keywords: | Trichinella spiralis, Cystatin, STAT4, Macrophage, IL-12, T-bet, IFN-gamma, Myotubule, L1 larval stage |
Divisions: | Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences |
Depositing User: | Symplectic Admin |
Date Deposited: | 28 Apr 2021 08:45 |
Last Modified: | 17 Oct 2023 05:05 |
DOI: | 10.1016/j.cellimm.2021.104303 |
Open Access URL: | https://doi.org/10.1016/j.cellimm.2021.104303 |
Related URLs: | |
URI: | https://livrepository.liverpool.ac.uk/id/eprint/3120850 |