Trichinella spiralis cystatin, TsCstN, modulates STAT4/IL-12 to specifically suppress IFN-gamma production



Kobpornchai, Porntida, Tiffney, Ellen-Alana, Adisakwattana, Poom and Flynn, Robin J ORCID: 0000-0001-5304-3088
(2021) Trichinella spiralis cystatin, TsCstN, modulates STAT4/IL-12 to specifically suppress IFN-gamma production. CELLULAR IMMUNOLOGY, 362. 104303-.

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Abstract

We have previously identified a cystatin, TsCstN, derived from the L1 stage of Trichinella spiralis and have shown that this protein is internalised in macrophages. Here we sought to address if this macrophage-TsCstN interaction could alter downstream T-cell priming. Using LPS-primed macrophages to stimulate T-cells in a co-culture system with or without TsCstN we assessed the resultant T-cell outcomes. IFN-γ, both protein and mRNA, but not IL-17A was negatively regulated by inclusion of TsCstN during macrophage priming. We identified a cell-cell contact independent change in the levels of IL-12 that led to altered phosphorylated STAT4 levels and translocation. TsCstN also negatively regulated the autonomous response in the myotubule cell line, C2C12. This work identifies a potential pathyway for L1 larvae to evade protective Th1 based immune responses and establish muscle-stage T. spiralis infection.

Item Type: Article
Uncontrolled Keywords: Trichinella spiralis, Cystatin, STAT4, Macrophage, IL-12, T-bet, IFN-gamma, Myotubule, L1 larval stage
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 28 Apr 2021 08:45
Last Modified: 18 Jan 2023 22:50
DOI: 10.1016/j.cellimm.2021.104303
Open Access URL: https://doi.org/10.1016/j.cellimm.2021.104303
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3120850