Cost effective optimised synthetic surface modification strategies for enhanced control of neuronal cell differentiation and supporting neuronal and Schwann cell viability

Taylor, Caroline S, Chen, Rui, D' Sa, Raechelle, Hunt, John A ORCID: 0000-0002-5168-4778, Curran, Judith M ORCID: 0000-0003-1551-2917 and Haycock, John W (2021) Cost effective optimised synthetic surface modification strategies for enhanced control of neuronal cell differentiation and supporting neuronal and Schwann cell viability. JOURNAL OF BIOMEDICAL MATERIALS RESEARCH PART B-APPLIED BIOMATERIALS, 109 (11). pp. 1713-1723.

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Official URL: http://dx.doi.org/10.1002/jbm.b.34829

Abstract

Enriching a biomaterial surface with specific chemical groups has previously been considered for producing surfaces that influence cell response. Silane layer deposition has previously been shown to control mesenchymal stem cell adhesion and differentiation. However, it has not been used to investigate neuronal or Schwann cell responses in vitro to date. We report on the deposition of aminosilane groups for peripheral neurons and Schwann cells studying two chain lengths: (a) 3-aminopropyl triethoxysilane (short chain-SC) and (b) 11-aminoundecyltriethoxysilane (long chain-LC) by coating glass substrates. Surfaces were characterised by water contact angle, AFM and XPS. LC-NH<sub>2</sub> was produced reproducibly as a homogenous surface with controlled nanotopography. Primary neuron and NG108-15 neuronal cell differentiation and primary Schwann cell responses were investigated in vitro by S100β, p75, and GFAP antigen expression. Both amine silane surface supported neuronal and Schwann cell growth; however, neuronal differentiation was greater on LC aminosilanes versus SC. Thus, we report that silane surfaces with an optimal chain length may have potential in peripheral nerve repair for the modification and improvement of nerve guidance devices.

Item Type:	Article
Uncontrolled Keywords:	amine, dorsal root ganglion, peripheral nerve injury, silane, surface chemistry, surface coating
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Life Courses and Medical Sciences Faculty of Science and Engineering > School of Engineering
Depositing User:	Symplectic Admin
Date Deposited:	11 May 2021 07:35
Last Modified:	18 Jan 2023 22:48
DOI:	10.1002/jbm.b.34829
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3122230