Harnessing the yeast <i>Saccharomyces cerevisiae</i> for the production of fungal secondary metabolites

Wang, Guokun, Kell, Douglas B ORCID: 0000-0001-5838-7963 and Borodina, Irina
(2021) Harnessing the yeast <i>Saccharomyces cerevisiae</i> for the production of fungal secondary metabolites. MICROBIAL CELL FACTORIES-BOOK, 65 (2). pp. 277-291.

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Fungal secondary metabolites (FSMs) represent a remarkable array of bioactive compounds, with potential applications as pharmaceuticals, nutraceuticals, and agrochemicals. However, these molecules are typically produced only in limited amounts by their native hosts. The native organisms may also be difficult to cultivate and genetically engineer, and some can produce undesirable toxic side-products. Alternatively, recombinant production of fungal bioactives can be engineered into industrial cell factories, such as aspergilli or yeasts, which are well amenable for large-scale manufacturing in submerged fermentations. In this review, we summarize the development of baker's yeast Saccharomyces cerevisiae to produce compounds derived from filamentous fungi and mushrooms. These compounds mainly include polyketides, terpenoids, and amino acid derivatives. We also describe how native biosynthetic pathways can be combined or expanded to produce novel derivatives and new-to-nature compounds. We describe some new approaches for cell factory engineering, such as genome-scale engineering, biosensor-based high-throughput screening, and machine learning, and how these tools have been applied for S. cerevisiae strain improvement. Finally, we prospect the challenges and solutions in further development of yeast cell factories to more efficiently produce FSMs.

Item Type: Article
Uncontrolled Keywords: Fungi, Saccharomyces cerevisiae, Metabolic Engineering
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 04 Jun 2021 14:05
Last Modified: 18 Oct 2023 18:23
DOI: 10.1042/EBC20200137
Open Access URL: https://portlandpress.com/essaysbiochem/article/do...
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URI: https://livrepository.liverpool.ac.uk/id/eprint/3125107