Novel Campylobacter concisus lipooligosaccharide is a determinant of inflammatory potential and virulence.

Brunner, Katja, John, Constance M, Phillips, Nancy J, Alber, Dagmar G, Gemmell, Matthew R ORCID: 0000-0002-2443-1986, Hansen, Richard ORCID: 0000-0002-3944-6646, Nielsen, Hans L, Hold, Georgina L ORCID: 0000-0001-7573-3397, Bajaj-Elliott, Mona ORCID: 0000-0002-5234-576X and Jarvis, Gary A ORCID: 0000-0002-7576-3702 (2018) Novel Campylobacter concisus lipooligosaccharide is a determinant of inflammatory potential and virulence. Journal of lipid research, 59 (10). pp. 1893-1905. ISSN 0022-2275, 1539-7262

Access the full-text of this item by clicking on the Open Access link.

Official URL: https://doi.org/10.1194/jlr.m085860

Abstract

The pathogenicity of Campylobacter concisus, increasingly found in the human gastrointestinal (GI) tract, is unclear. Some studies indicate that its role in GI conditions has been underestimated, whereas others suggest that the organism has a commensal-like phenotype. For the enteropathogen C. jejuni, the lipooligosaccharide (LOS) is a main driver of virulence. We investigated the LOS structure of four C. concisus clinical isolates and correlated the inflammatory potential of each isolate with bacterial virulence. Mass spectrometric analyses of lipid A revealed a novel hexa-acylated diglucosamine moiety with two or three phosphoryl substituents. Molecular and fragment ion analysis indicated that the oligosaccharide portion of the LOS had only a single phosphate and lacked phosphoethanolamine and sialic acid substitution, which are hallmarks of the C. jejuni LOS. Consistent with our structural findings, C. concisus LOS and live bacteria induced less TNF-α secretion in human monocytes than did C. jejuni Furthermore, the C. concisus bacteria were less virulent than C. jejuni in a Galleria mellonella infection model. The correlation of the novel lipid A structure, decreased phosphorylation, and lack of sialylation along with reduced inflammatory potential and virulence support the significance of the LOS as a determinant in the relative pathogenicity of C. concisus.

Item Type:	Article
Uncontrolled Keywords:	Cell Line, Humans, Campylobacter, Inflammation, Lipopolysaccharides, Lipid A, Tumor Necrosis Factor-alpha, Genomics, Virulence, Signal Transduction
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	07 Jun 2021 09:40
Last Modified:	07 Dec 2024 05:06
DOI:	10.1194/jlr.m085860
Open Access URL:	https://doi.org/10.1194/jlr.M085860
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3125423