Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms



Omileke, Daniel, Bothwell, Steven, Beard, Daniel J, Mackovski, Nikolce, Azarpeykan, Sara, Coupland, Kirsten, Patabendige, Adjanie ORCID: 0000-0003-1550-3084 and Spratt, Neil
(2021) Short-Duration Hypothermia Induction in Rats using Models for Studies examining Clinical Relevance and Mechanisms. JOVE-JOURNAL OF VISUALIZED EXPERIMENTS, 2021 (169). pp. 1-10.

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Abstract

Therapeutic hypothermia (TH) is a powerful neuroprotective strategy that has provided robust evidence for neuroprotection in pre-clinical studies of neurological disorders. Despite strong pre-clinical evidence, TH has not shown efficacy in clinical trials of most neurological disorders. The only successful trials employing therapeutic hypothermia were related to cardiac arrest in adults and hypoxic ischemic injury in neonates. Further investigations into the parameters of its use, and study design comparisons between pre-clinical and clinical studies, are warranted. This article demonstrates two methods of short-duration hypothermia induction. The first method allows for rapid hypothermia induction in rats using ethanol spray and fans. This method works by cooling the skin, which has been less commonly used in clinical trials and may have different physiological effects. Cooling is much more rapid with this technique than is achievable in human patients due to differences in surface area to volume ratio. Along with this, a second method is also presented, which allows for a clinically achievable cooling rate for short-duration hypothermia. This method is easy to implement, reproducible and does not require active skin cooling.

Item Type: Article
Uncontrolled Keywords: Skin, Animals, Rats, Rats, Wistar, Hypothermia, Induced, Models, Animal, Time Factors, Male, Cold Temperature
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User: Symplectic Admin
Date Deposited: 10 Jun 2021 08:59
Last Modified: 02 Mar 2024 09:45
DOI: 10.3791/62325
Open Access URL: https://dx.doi.org/10.3791/62325
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3125843