Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients

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Poh, Chek Meng, Carissimo, Guillaume, Wang, Bei, Amrun, Siti Naqiah, Lee, Cheryl Yi-Pin, Chee, Rhonda Sin-Ling, Fong, Siew-Wai, Yeo, Nicholas Kim-Wah, Lee, Wen-Hsin, Torres-Ruesta, Anthony et al (show 13 more authors) , Leo, Yee-Sin, Chen, Mark I-Cheng, Tan, Seow-Yen, Chai, Louis Yi Ann, Kalimuddin, Shirin, Kheng, Shirley Seah Gek, Thien, Siew-Yee, Young, Barnaby Edward, Lye, David C, Hanson, Brendon John, Wang, Cheng-I, Renia, Laurent and Ng, Lisa FP ORCID: 0000-0003-4071-5222 (2020) Two linear epitopes on the SARS-CoV-2 spike protein that elicit neutralising antibodies in COVID-19 patients. NATURE COMMUNICATIONS, 11 (1). 2806-.

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Abstract

Given the ongoing SARS-CoV-2 pandemic, identification of immunogenic targets against the coronavirus spike glycoprotein will provide crucial advances towards the development of sensitive diagnostic tools and potential vaccine candidate targets. In this study, using pools of overlapping linear B-cell peptides, we report two IgG immunodominant regions on SARS-CoV-2 spike glycoprotein that are recognised by sera from COVID-19 convalescent patients. Notably, one is specific to SARS-CoV-2, which is located in close proximity to the receptor binding domain. The other region, which is localised at the fusion peptide, could potentially function as a pan-SARS target. Functionally, antibody depletion assays demonstrate that antibodies targeting these immunodominant regions significantly alter virus neutralisation capacities. Taken together, identification and validation of these neutralising B-cell epitopes will provide insights towards the design of diagnostics and vaccine candidates against this high priority coronavirus.

Item Type:	Article
Uncontrolled Keywords:	Humans, Pneumonia, Viral, Coronavirus Infections, Immunoglobulin G, Antibodies, Viral, Epitopes, B-Lymphocyte, Immunodominant Epitopes, Amino Acid Sequence, Antibodies, Neutralizing, Pandemics, Spike Glycoprotein, Coronavirus, Betacoronavirus, COVID-19, SARS-CoV-2
Divisions:	Faculty of Health and Life Sciences Faculty of Health and Life Sciences > Institute of Infection, Veterinary and Ecological Sciences
Depositing User:	Symplectic Admin
Date Deposited:	17 Jun 2021 09:18
Last Modified:	18 Jan 2023 22:34
DOI:	10.1038/s41467-020-16638-2
Open Access URL:	http://doi.org/10.1038/s41467-020-16638-2
Related URLs:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3126701