Exploring the fitness landscape of gastroenteritis-associated and bloodstream infection-associated Salmonella Enteritidis



Fong, Wai Yee
(2021) Exploring the fitness landscape of gastroenteritis-associated and bloodstream infection-associated Salmonella Enteritidis. PhD thesis, University of Liverpool.

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Abstract

Non-typhoidal Salmonella (NTS) typically causes gastroenteritis in humans. However, in recent years NTS have emerged as a major causative agent of bloodstream infections in sub Saharan Africa, with Salmonella Enteritidis ranked as the second most common serovar associated with this invasive disease. Genomic and phylogenetic characterisation of S. Enteritidis isolates from human bloodstream has identified the Central/Eastern African clade (CEAC) and Western African clade that are significantly different to the gastroenteritis-associated Global Epidemic clade (GEC). The African clades have distinct genetic features such as genomic degradation, novel prophage repertoires and multi-drug resistance. The molecular basis for the enhanced propensity of African S. Enteritidis to cause bloodstream infection remains poorly understood. This thesis explores the molecular basis for African S. Enteritidis virulence, by investigating the genetic determinants of the GEC-representative strain P125109 and the CEAC-representative strain D7795 for growth in vitro in LB, NonSPI2 and InSPI2 conditions, and replication in RAW 264.7 murine macrophages. Two functional genomic approaches, RNA sequencing (RNA-seq) and transposon insertion ... (continues)

Item Type: Thesis (PhD)
Divisions: Faculty of Health and Life Sciences
Depositing User: Symplectic Admin
Date Deposited: 10 Sep 2021 10:03
Last Modified: 08 Aug 2024 10:39
DOI: 10.17638/03129829
Supervisors:
  • Hinton, Jay CD
  • Wigley, Paul
URI: https://livrepository.liverpool.ac.uk/id/eprint/3129829