Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers

Robinson, Christopher J, Carbonell, Pablo, Jervis, Adrian J, Yan, Cunyu, Hollywood, Katherine A, Dunstan, Mark S, Currin, Andrew, Swainston, Neil ORCID: 0000-0001-7020-1236, Spiess, Reynard, Taylor, Sandra
et al (show 13 more authors) (2020) Rapid prototyping of microbial production strains for the biomanufacture of potential materials monomers. METABOLIC ENGINEERING, 60. pp. 168-182.

Access the full-text of this item by clicking on the Open Access link.


Bio-based production of industrial chemicals using synthetic biology can provide alternative green routes from renewable resources, allowing for cleaner production processes. To efficiently produce chemicals on-demand through microbial strain engineering, biomanufacturing foundries have developed automated pipelines that are largely compound agnostic in their time to delivery. Here we benchmark the capabilities of a biomanufacturing pipeline to enable rapid prototyping of microbial cell factories for the production of chemically diverse industrially relevant material building blocks. Over 85 days the pipeline was able to produce 17 potential material monomers and key intermediates by combining 160 genetic parts into 115 unique biosynthetic pathways. To explore the scale-up potential of our prototype production strains, we optimized the enantioselective production of mandelic acid and hydroxymandelic acid, achieving gram-scale production in fed-batch fermenters. The high success rate in the rapid design and prototyping of microbially-produced material building blocks reveals the potential role of biofoundries in leading the transition to sustainable materials production.

Item Type: Article
Uncontrolled Keywords: Synthetic biology, Biofoundry, Biomanufacturing, Industrial biotechnology, Mandelic acid, Hydroxymandelic acid
Divisions: Faculty of Health and Life Sciences
Faculty of Health and Life Sciences > Institute of Systems, Molecular and Integrative Biology
Depositing User: Symplectic Admin
Date Deposited: 13 Jul 2021 08:26
Last Modified: 23 Feb 2023 05:19
DOI: 10.1016/j.ymben.2020.04.008
Open Access URL: https://doi.org/10.1016/j.ymben.2020.04.008
Related URLs:
URI: https://livrepository.liverpool.ac.uk/id/eprint/3129862