Untargeted fecal metabolome analysis in obese dogs after weight loss achieved by feeding a high-fiber-high-protein diet

Bermudez Sanchez, Sandra, Pilla, Rachel, Sarawichitr, Benjamin, Gramenzi, Alessandro, Marsilio, Fulvio, Steiner, Joerg M, Lidbury, Jonathan A, Woods, Georgiana RT, Suchodolski, Jan S and German, Alexander J ORCID: 0000-0002-3017-7988 (2021) Untargeted fecal metabolome analysis in obese dogs after weight loss achieved by feeding a high-fiber-high-protein diet METABOLOMICS, 17 (7). 66-. ISSN 1573-3882, 1573-3890

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Abstract

Introduction: In humans and companion animals, obesity is accompanied by metabolic derangements. Studies have revealed differences in the composition of the fecal microbiome between obese dogs and those with an ideal body weight. Objectives: We have previously reported that the fecal microbiome in obese dogs changes after controlled weight reduction, induced by feeding a diet high in fiber and protein. Despite these findings, it is unclear if taxonomic differences infer differences at the functional level between obese dogs and those with an ideal body weight. Methodology: Untargeted fecal metabolome analysis was performed on dogs with obesity before and after weight loss achieved by feeding a high-fiber-high-protein diet. Results: Fecal metabolome analysis revealed a total of 13 compounds that changed in concentration in obese dogs after weight loss. Of these compounds, metabolites associated with bacterial metabolism decreased after weight loss including purine, L-(-)-methionine, coumestrol, and the alkaloids 1-methylxanthine and trigonelline. Conversely, the polyphenols (-)-epicatechin and matairesinol and the quinoline derivatives 1,5-isoquinolinediol and 2-hydroxiquinoline increased after weight loss. Conclusion: These results suggest differences in intestinal microbiome at the functional level after weight loss, but further studies are needed to determine the role of these compounds in the etiology of obesity and weight loss.

Item Type:	Article
Uncontrolled Keywords:	Canine obesity, Fecal microbiota, Fecal metabolome, Weight loss, Untargeted metabolome analysis
Divisions:	Faculty of Health & Life Sciences Faculty of Health & Life Sciences > Inst. Life Courses & Medical Sciences
Depositing User:	Symplectic Admin
Date Deposited:	13 Jul 2021 12:46
Last Modified:	22 May 2026 14:50
DOI:	10.1007/s11306-021-01815-1
Open Access URL:	https://link.springer.com/content/pdf/10.1007/s113...
Related Websites:	Author Publisher
URI:	https://livrepository.liverpool.ac.uk/id/eprint/3129878
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